Succession and determinants of the early life nasopharyngeal microbiota in a South African birth cohort.
Succession and determinants of the early life nasopharyngeal microbiota in a South African birth cohort.
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DOI：
10.1186/s40168-023-01563-5
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发表时间：
2023-06-05
期刊：
影响因子：
15.5
通讯作者：
中科院分区：
文献类型：
--
作者：
鼻咽部的细菌作为呼吸道健康的守门人发挥着关键作用。然而，在低收入和中等收入国家（LMIC），早期鼻咽（NP）细菌谱的动态仍然研究不足，这些国家的儿童下呼吸道感染的风险因素患病率很高。我们调查了南非低收入家庭健康婴儿NP细菌谱的纵向变化和相关暴露。我们使用短片段（V4区）16 S rRNA基因扩增子测序来表征南非出生队列中103名婴儿的NP细菌谱，从出生到生命的前12个月每月一次，此后每6个月一次，直到30个月。棒状杆菌和葡萄球菌在1个月的生活占主导地位的殖民者，然而，这些迅速取代莫拉菌或嗜血杆菌占主导地位的配置文件4个月。这种继承几乎是普遍的，在很大程度上独立于广泛的曝光。温暖的天气（夏季）、较低的胎龄、母亲吸烟、无日托、抗生素暴露或12个月时年龄别身高z评分低与较高的α和β多样性相关。夏季也与较高的相对丰度的葡萄球菌，链球菌，奈瑟氏球菌，或厌氧革兰氏阴性菌，而春季和冬季分别与较高的相对丰度的嗜血杆菌或棒状杆菌。母亲吸烟与较高的相对丰度的卟啉单胞菌。抗生素治疗（或异烟肼预防结核病）与较高的相对丰度的厌氧类群（卟啉单胞菌，梭杆菌，和普氏菌）和较低的相对丰度的健康相关类群棒状杆菌和Dolosigranulum。艾滋病毒暴露与较高的相对丰度的克雷伯氏菌或韦荣氏菌和较低的相对丰度的一个未分类的属在家庭毛螺菌。在这个密集采样的队列中，有快速和可预测的替代早期配置文件为主的健康相关的棒状杆菌和Dolosigranulum与那些为主的莫拉氏菌和嗜血杆菌，独立的曝光。季节和抗生素暴露是NP细菌谱的关键决定因素。研究不足，但在中低收入国家，包括母亲吸烟和艾滋病毒暴露，流行的高度流行的风险，与NP细菌配置文件。视频摘要在线版本包含补充材料，可通过10. 1186/s40168-023-01563-5获取。
Bacteria colonizing the nasopharynx play a key role as gatekeepers of respiratory health. Yet, dynamics of early life nasopharyngeal (NP) bacterial profiles remain understudied in low- and middle-income countries (LMICs), where children have a high prevalence of risk factors for lower respiratory tract infection. We investigated longitudinal changes in NP bacterial profiles, and associated exposures, among healthy infants from low-income households in South Africa. We used short fragment (V4 region) 16S rRNA gene amplicon sequencing to characterize NP bacterial profiles from 103 infants in a South African birth cohort, at monthly intervals from birth through the first 12 months of life and six monthly thereafter until 30 months. Corynebacterium and Staphylococcus were dominant colonizers at 1 month of life; however, these were rapidly replaced by Moraxella- or Haemophilus-dominated profiles by 4 months. This succession was almost universal and largely independent of a broad range of exposures. Warm weather (summer), lower gestational age, maternal smoking, no day-care attendance, antibiotic exposure, or low height-for-age z score at 12 months were associated with higher alpha and beta diversity. Summer was also associated with higher relative abundances of Staphylococcus, Streptococcus, Neisseria, or anaerobic gram-negative bacteria, whilst spring and winter were associated with higher relative abundances of Haemophilus or Corynebacterium, respectively. Maternal smoking was associated with higher relative abundances of Porphyromonas. Antibiotic therapy (or isoniazid prophylaxis for tuberculosis) was associated with higher relative abundance of anerobic taxa (Porphyromonas, Fusobacterium, and Prevotella) and with lower relative abundances of health associated-taxa Corynebacterium and Dolosigranulum. HIV-exposure was associated with higher relative abundances of Klebsiella or Veillonella and lower relative abundances of an unclassified genus within the family Lachnospiraceae. In this intensively sampled cohort, there was rapid and predictable replacement of early profiles dominated by health-associated Corynebacterium and Dolosigranulum with those dominated by Moraxella and Haemophilus, independent of exposures. Season and antibiotic exposure were key determinants of NP bacterial profiles. Understudied but highly prevalent exposures prevalent in LMICs, including maternal smoking and HIV-exposure, were associated with NP bacterial profiles. Video Abstract The online version contains supplementary material available at 10.1186/s40168-023-01563-5.
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影响因子：
3.7
作者：
Clooney AG;Fouhy F;Sleator RD;O' Driscoll A;Stanton C;Cotter PD;Claesson MJ
通讯作者：
Claesson MJ
影响因子：
11.1
作者：
Bosch AATM;Levin E;van Houten MA;Hasrat R;Kalkman G;Biesbroek G;de Steenhuijsen Piters WAA;de Groot PCM;Pernet P;Keijser BJF;Sanders EAM;Bogaert D
通讯作者：
Bogaert D
在幼儿进行上呼吸道和下呼吸道感染的细菌之间的关联：系统评价和荟萃分析。
DOI：
10.1016/j.cmi.2021.05.034
发表时间：
2021-09
期刊：
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
影响因子：
--
作者：
Claassen-Weitz S;Lim KYL;Mullally C;Zar HJ;Nicol MP
通讯作者：
Nicol MP
影响因子：
4.2
作者：
Brooks JP;Edwards DJ;Harwich MD Jr;Rivera MC;Fettweis JM;Serrano MG;Reris RA;Sheth NU;Huang B;Girerd P;Vaginal Microbiome Consortium;Strauss JF 3rd;Jefferson KK;Buck GA
通讯作者：
Buck GA
影响因子：
2.4
作者：
Fenton TR;Kim JH
通讯作者：
Kim JH