Molecular cloning and characterization of four heat shock protein genes from Macrocentrus cingulum (Hymenoptera: Braconidae)
Molecular cloning and characterization of four heat shock protein genes from Macrocentrus cingulum (Hymenoptera: Braconidae)
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Macrocentrus cingulum（膜翅目：茧蜂科）四个热休克蛋白基因的分子克隆和表征
DOI：
10.1007/s11033-009-9715-z
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发表时间：
2010-06
影响因子：
2.8
通讯作者：
L. Liu
中科院分区：
文献类型：
--
作者：
J. Xiao;D. Huang;P. Xu;T. Li;L. Liu
从Macrocentrus cingulum中扩增出4个HSPs基因的全长cDNA序列，分别命名为Mchsp90、Mchsc70、Mchsp70和Mchsp23.8。这四个基因被提交到GenBank数据库并分配给GenBank登录号：EU570065、EU585780、EU585779和EU624206。系统发育分析表明Mchsp90、Mchsc70和Mchsp70位于细胞质中。此外，Mchsc70是热休克同源蛋白70的成员，而Mchsp70是热休克诱导蛋白70的成员。表达分析表明，这4个基因在幼虫、蛹和成虫阶段存在差异表达。正常情况下，Mchsp90和Mchsc70的mRNA水平高于Mchsp70，而Mchsp23.8的mRNA水平随着衰老而降低。除了Mchsp70和Mchsp23.8之外，这4个基因的mRNA水平在热休克的作用下也能上调，而Mchsp90和Mchsc70的增加幅度更大。这些结果表明Mchsp90、Mchsp70和Mchsp23.8可能在正常和热激条件下具有双重功能，而Mchsp70和Mchsp23.8可能在热激条件下提供更重要的保护。
The full-length cDNA sequences of four HSPs genes were amplified from Macrocentrus cingulum, respectively named Mchsp90, Mchsc70, Mchsp70 and Mchsp23.8. These four genes were submitted to GenBank database and assigned to GenBank accession number: EU570065, EU585780, EU585779 and EU624206. Phylogenetic analysis indicated that the Mchsp90, Mchsc70 and Mchsp70 were located in cytoplasm. Moreover, the Mchsc70 was a member of heat shock cognate protein 70 while the Mchsp70 was a member of heat shock inducible 70. The expression analysis indicated that these four genes were differentially expressed at larval, pupal and adult stages. Under normal conditions, the mRNA levels of Mchsp90 and Mchsc70 were higher than Mchsp70 while Mchsp23.8 was decreased during aging. The mRNA levels of these four genes could be up-regulated by heat shock besides Mchsp70 and Mchsp23.8 could be increased more dramatically than Mchsp90 and Mchsc70. These results suggested that Mchsp90, Mchsp70 and Mchsp23.8 might have dual functions under normal and heat shock conditions while Mchsp70 and Mchsp23.8 might supply more important protection under heat shock.
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影响因子：
5.3
作者：
Demand, J;Lüders, J;Höhfeld, J
通讯作者：
Höhfeld, J
影响因子：
5.3
作者：
E. Hickey;S. E. Brandon;G. Smale;D. Lloyd;L. Weber
通讯作者：
E. Hickey;S. E. Brandon;G. Smale;D. Lloyd;L. Weber
影响因子：
1.2
作者：
R. Wu;Y. Sun;L. Lei;S. Xie
通讯作者：
R. Wu;Y. Sun;L. Lei;S. Xie
DOI：
--
发表时间：
1984
期刊：
--
影响因子：
--
作者：
G. Malacinski;S. Bryant
通讯作者：
G. Malacinski;S. Bryant
影响因子：
2.2
作者：
Edwards, OR;Hopper, KR
通讯作者：
Hopper, KR