Genetic context of bla (CTX-M-55) and qnrS1 genes in a foodborne Salmonella enterica serotype Saintpaul isolate from China.
Genetic context of bla (CTX-M-55) and qnrS1 genes in a foodborne Salmonella enterica serotype Saintpaul isolate from China.
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来自中国的食源性肠沙门氏菌血清型 Saintpaul 分离物中 blaCTX-M-55 和 qnrS1 基因的遗传背景
DOI：
10.3389/fmicb.2022.899062
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
耐氟喹诺酮类(FQS)和超广谱头孢菌素类(ESCs)的肠炎沙门氏菌已被世界卫生组织列为重点病原菌。肠炎沙门氏菌Saintpaul(S.Saintpaul)对ESCs和FQS共耐药，并携带相应的耐药基因(blaCTX-M-55和qnrS1)。然而，在中国中还没有报道过。此外，圣保罗葡萄球菌ESCS和FQS抗性基因的遗传背景和可转移性仍然不清楚。本研究首次对中国地区一株具有多重耐药基因的圣保罗葡萄球菌(16Sal016)进行了鉴定，该菌株携带了从中国网织鱼中回收的blaCTX-M-55和qnrS1基因。全基因组短读和长读测序结果表明，在259,529个碱基的IncHI2上存在15个编码对9类抗生素的耐药性的获得性抗生素耐药基因，以及丰富的可移动遗传元件。BlaCTX-M-55和qnrS1基因位于12,865个碱基区，IS26-orf-orf-ISKpn19-qnrS1-IS3-Tn3-orf-blaCTX–M–55-ISEc9-orf-IS26.在不同的细菌物种中发现了相似的结构，表明blaCTX-M-55和qnrS1基因在该基因簇中具有很高的可转移性。结果表明，该重组质粒可通过接合方式转移到大肠杆菌J53中，并可获得多重抗性。基于全球2947株圣保罗葡萄球菌的核心基因组多位点序列分型(CgMLST)比较表明，16Sal016菌株与英国的一株分离物在流行病学上是连锁的。我们的发现表明，质粒和IS26介导的移动遗传元件是圣保罗葡萄球菌blaCTX-M-55和qnrS1基因的携带者，并突出了它们的潜在传播，这需要继续研究。
Salmonella enterica resistant to fluoroquinolones (FQs) and extended-spectrum cephalosporins (ESCs) has been deemed a high-priority pathogen by the WHO. Salmonella enterica serovar Saintpaul (S. Saintpaul) co-resistant to ESCs and FQs and harboring corresponding resistance genes (blaCTX–M–55 and qnrS1) have been previously reported. However, they have not been reported in China. Moreover, the genetic context and transferability of ESCs and FQs resistance genes in S. Saintpaul remain obscure. This study is the first study to characterize a multidrug-resistant (MDR) S. Saintpaul isolate (16Sal016) harboring plasmid-mediated blaCTX–M–55 and qnrS1 genes recovered from weever fish in China. The whole genome short- and long-read sequencing results identified the presence of 15 acquired antibiotic resistance genes encoding resistance to nine classes of antibiotics, as well as abundant mobile genetic elements residing on a 259,529 bp IncHI2 plasmid. The blaCTX–M–55 and qnrS1 genes were located in a 12,865 bp region, IS26-orf-orf-ISKpn19-qnrS1-IS3-Tn3-orf-blaCTX–M–55-ISEc9-orf-IS26. Similar structures have been identified in various bacterial species, indicating a high transferability of blaCTX–M–55 and qnrS1 genes within this gene cluster. The plasmid was found to be transferable to Escherichia coli (E. coli) J53 by conjugation and resulted in the acquisition of multiple resistances by the transconjugants. Genome sequence comparisons by core genome multilocus sequence typing (cgMLST) based on global 2,947 S. Saintpaul isolates indicated that strain 16Sal016 was epidemiologically linked with an isolate from the United Kingdom (UK). Our findings suggest that plasmids and IS26-mediated mobile genetic elements are carriers of blaCTX–M–55 and qnrS1 genes in S. Saintpaul, and highlight their potential transmission, which needs continuous investigations.
影响因子：
4.2
作者：
Ding Y;Zwe YH;Chin SF;Kohli GS;Drautz-Moses DI;Givskov M;Schlundt J;Schuster SC;Yuk HG;Yang L
通讯作者：
Yang L