Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul.
Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul.
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DOI：
10.1186/s13099-018-0249-6
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发表时间：
2018
期刊：
影响因子：
4.2
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Ding Y;Zwe YH;Chin SF;Kohli GS;Drautz-Moses DI;Givskov M;Schlundt J;Schuster SC;Yuk HG;Yang L
肠道沙门氏菌亚种圣保罗沙门氏菌（S. Saintpaul）是引起沙门氏菌病的重要肠道病原体。虽然肠道沙门氏菌病通常是自限性的，但它对儿童、老年人和免疫功能低下的患者可能会危及生命。因此，需要对这些患者进行适当的抗生素治疗。然而，由于多药耐药（MDR）质粒的传播，许多抗生素对肠链球菌感染的疗效已大大降低，对公共卫生构成严重威胁，需要密切监测。在这项研究中，我们对从鸡中分离的肠链球菌耐多药质粒pSGB23进行了测序和完全表征。全基因组序列分析显示，S. Saintpaul菌株SGB23含有一个254 kb的巨质粒pSGB23，该质粒携带11个抗生素耐药基因，对9类抗生素和常用的食品加工设备消毒用季铵化合物具有耐药性。此外，我们发现pSGB23携带多个共轭系统，使其能够通过自偶联传播到其他肠杆菌科细菌中。它还拥有多种类型的复制子和质粒维持和成瘾系统，这解释了它的宿主范围广泛和遗传稳定。我们在此报告了一种由肠球菌携带的新型耐多药质粒pSGB23。据我们所知，它是迄今发现的肠链球菌耐多药质粒中携带抗生素耐药基因最多、耐药谱范围最广的。从食物来源中分离出的pSGB23令人担忧，将根据本研究报告的结果对其进一步传播进行监测。
Salmonella enterica subspecies enterica serovar Saintpaul (S. Saintpaul) is an important gut pathogen which causes salmonellosis worldwide. Although intestinal salmonellosis is usually self-limiting, it can be life-threatening in children, the elderlies and immunocompromised patients. Appropriate antibiotic treatment is therefore required for these patients. However, the efficacy of many antibiotics on S. enterica infections has been greatly compromised due to spreading of multidrug resistance (MDR) plasmids, which poses serious threats on public health and needs to be closely monitored. In this study, we sequenced and fully characterized an S. enterica MDR plasmid pSGB23 isolated from chicken. Complete genome sequence analysis revealed that S. Saintpaul strain SGB23 harbored a 254 kb megaplasmid pSGB23, which carries 11 antibiotic resistance genes responsible for resistance to 9 classes of antibiotics and quaternary ammonium compounds that are commonly used to disinfect food processing facilities. Furthermore, we found that pSGB23 carries multiple conjugative systems, which allow it to spread into other Enterobacteriaceae spp. by self-conjugation. It also harbors multiple types of replicons and plasmid maintenance and addictive systems, which explains its broad host range and stable inheritance. We report here a novel MDR plasmid pSGB23 harboured by S. enterica. To our knowledge, it carried the greatest number of antibiotic resistance genes with the broadest range of resistance spectrum among S. enterica MDR plasmids identified so far. The isolation of pSGB23 from food sources is worrisome, while surveillance on its further spreading will be carried out based on the findings reported in this study.
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