Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.
Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.
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培养的小鼠原代骨骼肌管中肌原纤维组装过程中肌节蛋白的定位。
DOI：
10.1002/ar.22981
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Sanger,JeanM
中科院分区：
文献类型：
--
作者：
White,Jennifer;Barro,MariettaV;Makarenkova,HelenP;Sanger,JosephW;Sanger,JeanM
It is important to understand how muscle forms normally in order to understand muscle diseases that result in abnormal muscle formation. Although the structure of myofibrils is well understood, the process through which the myofibril components form organized contractile units is not clear. Based on the staining of muscle proteins in avian embryonic cardiomyocytes, we previously proposed that myofibrils formation occurred in steps that began with premyofibrils followed by nascent myofibrils and ending with mature myofibrils. The purpose of this study was to determine whether the premyofibril model of myofibrillogenesis developed from studies developed from studies in avian cardiomyocytes was supported by our current studies of myofibril assembly in mouse skeletal muscle. Emphasis was on establishing how the key sarcomeric proteins, F‐actin, nonmuscle myosin II, muscle myosin II, and α‐actinin were organized in the three stages of myofibril assembly. The results also test previous reports that nonmuscle myosins II A and B are components of the Z‐bands of mature myofibrils, data that are inconsistent with the premyofibril model. We have also determined that in mouse muscle cells, telethonin is a late assembling protein that is present only in the Z‐bands of mature myofibrils. This result of using specific telethonin antibodies supports the approach of using YFP‐tagged proteins to determine where and when these YFP‐sarcomeric fusion proteins are localized. The data presented in this study on cultures of primary mouse skeletal myocytes are consistent with the premyofibril model of myofibrillogenesis previously proposed for both avian cardiac and skeletal muscle cells. Anat Rec, 297:1571–1584, 2014. © 2014 Wiley Periodicals, Inc.
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二酰甘油对蛋白激酶C激活特异性的进一步研究。
DOI：
--
发表时间：
1987
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
M. Go;K. Sekiguchi;H. Nomura;U. Kikkawa;Y. Nishizuka
通讯作者：
Y. Nishizuka
来自大鼠脑的钙激活、磷脂依赖性蛋白激酶。
影响因子：
4.8
作者：
U. Kikkawa;Y. Takai;R. Minakuchi;S. Inohara;Y. Nishizuka
通讯作者：
Y. Nishizuka
DOI：
10.1016/0006-291x(86)90754-0
发表时间：
1986-11-14
影响因子：
3.1
作者：
NOMURA, H;ASE, K;SATOH, T
通讯作者：
SATOH, T
DOI：
10.1016/s0021-9258(18)33173-9
发表时间：
1983-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Akira Kishimoto;Norio Kajikawag;Makoto Shiotan;Yasutomi Nishizuka
通讯作者：
Akira Kishimoto;Norio Kajikawag;Makoto Shiotan;Yasutomi Nishizuka
肿瘤启动子介导的蛋白激酶 C 激活的进一步表征。
DOI：
--
发表时间：
1984
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
A. Couturier;S. Bazgar;M. Castagna
通讯作者：
M. Castagna