Airway CD8(+) T cells induced by pulmonary DNA immunization mediate protective anti-viral immunity.
Airway CD8(+) T cells induced by pulmonary DNA immunization mediate protective anti-viral immunity.
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作者：
预防多种呼吸道病原体的疫苗接种策略必须诱导肺气道和外周淋巴器官中的 T 细胞群。在这项研究中，我们表明，使用由聚合物聚乙烯亚胺 (PEI-DNA) 配制的质粒 DNA 进行肺部免疫可在气道中诱导抗原特异性 CD8+ T 细胞，这些细胞在抗原局部清除后仍能长期存在。细胞的持续存在不是由局部淋巴细胞增殖或肺或气道内持续抗原呈递介导的。这些疫苗诱导的 CD8+ T 细胞有效介导针对牛痘病毒和流感病毒呼吸道攻击的保护性免疫。此外，这种保护并不依赖于从外周部位招募 T 细胞。这些发现表明，使用 PEI-DNA 进行肺部免疫是诱导强大的肺部 CD8+ T 细胞群的有效方法，可有效预防呼吸道病原体。
Vaccination strategies for protection against a number of respiratory pathogens must induce T-cell populations in both the pulmonary airways and peripheral lymphoid organs. In this study, we show that pulmonary immunization using plasmid DNA formulated with the polymer polyethyleneimine (PEI-DNA) induced antigen-specific CD8+ T cells in the airways that persisted long after antigen local clearance. The persistence of the cells was not mediated by local lymphocyte proliferation or persistent antigen presentation within the lung or airways. These vaccine-induced CD8+ T cells effectively mediated protective immunity against respiratory challenges with vaccinia virus and influenza virus. Moreover, this protection was not dependent upon the recruitment of T cells from peripheral sites. These findings demonstrate that pulmonary immunization with PEI-DNA is an efficient approach for inducing robust pulmonary CD8+ T-cell populations that are effective at protecting against respiratory pathogens.
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DOI：
10.1084/jem.184.5.1879
发表时间：
1996-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gallichan WS;Rosenthal KL
通讯作者：
Rosenthal KL
影响因子：
3.7
作者：
Bonnet, Marie-Elise;Erbacher, Patrick;Bolcato-Bellemin, Anne-Laure
通讯作者：
Bolcato-Bellemin, Anne-Laure
影响因子：
4.4
作者：
Hogan, RJ;Usherwood, EJ;Woodland, DL
通讯作者：
Woodland, DL
影响因子：
32.4
作者：
Kohlmeier JE;Cookenham T;Roberts AD;Miller SC;Woodland DL
通讯作者：
Woodland DL
影响因子：
5.4
作者：
Perrone, Lucy A.;Ahmad, Attiya;Tumpey, Terrence M.
通讯作者：
Tumpey, Terrence M.