Exogenous putrescine alleviates photoinhibition caused by salt stress through cooperation with cyclic electron flow in cucumber
Exogenous putrescine alleviates photoinhibition caused by salt stress through cooperation with cyclic electron flow in cucumber
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外源腐胺通过与黄瓜循环电子流的配合减轻盐胁迫引起的光抑制
DOI：
10.1007/s11120-019-00631-y
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发表时间：
2019-04
影响因子：
3.7
通讯作者：
Guo Shirong
中科院分区：
文献类型：
--
作者：
Wu Xinyi;Shu Sheng;Wang Yu;Yuan Ruonan;Guo Shirong
当植物遭受非生物胁迫时，循环电子流(CEF)被诱导以起到光保护作用。腐胺(Put)是叶绿体中的一种主要多胺，在逆境耐受性中起着关键作用。然而，CEF和Put在叶绿体中用于光保护的关系尚不清楚。在本研究中，我们研究了Put诱导的CEF在黄瓜耐盐性中的作用。90 mM氯化钠和/或PUT处理不影响PSII的最大光化学效率(Fv/Fm)，但盐严重抑制了PSI的光活性。盐胁迫诱导了较高水平的CEF，而且与单盐处理相比，盐和Put同时处理的植株表现出更高的CEF活性和ATP积累，从而为植物的生长提供了合适的ATP/NADPH比值。此外，PUT降低了跨膜质子梯度(ΔpH)，并伴随着pH依赖的非光化学猝灭(NPQ)的减少，增加了PSII(Y(II))的有效量子产率。与盐处理相比，盐胁迫叶片NADP+/NADPH比值显著增加，说明Put缓解了盐胁迫引起的PSI受体侧的超还原压力。综上所述，我们的结果表明，外源Put为促进CEF创造了良好的条件：大量的PMF主要以Δψ的形式储存，导致管腔pH适中和低NPQ，同时保持高的ATP合成速率(高PMF)。
When plants suffer from abiotic stresses, cyclic electron flow (CEF) is induced for photo-protection. Putrescine (Put), a primary polyamine in chloroplasts, plays a critical role in stress tolerance. However, the relationship between CEF and Put in chloroplasts for photo-protection is unknown. In this study, we investigated the role of Put-induced CEF for salt tolerance in cucumber plants (Cucumis sativusL). Treatment with 90 mM NaCl and/or Put did not influence the maximum photochemical efficiency of PSII (Fv/Fm), but the photoactivity of PSI was severely inhibited by NaCl. Salt stress induced a high level of CEF; moreover, plants treated with both NaCl and Put exhibited much higher CEF activity and ATP accumulation than those exhibited by single-salt-treated plants to provide an adequate ATP/NADPH ratio for plant growth. Furthermore, Put decreased the trans-membrane proton gradient (ΔpH), which was accompanied by reduced pH-dependent non-photochemical quenching (NPQ) and an increased the effective quantum yield of PSII (Y(II)). The ratio of NADP+/NADPH increased significantly with Put in salt-stressed leaves compared with the ratio in leaves treated with NaCl, indicating that Put relieved over-reduction pressure at the PSI acceptor side caused by salt stress. Collectively, our results suggest that exogenous Put creates an excellent condition for CEF promotion: a large amount ofpmfis predominantly stored as Δψ, resulting in moderate lumen pH and low NPQ, while maintaining high rates of ATP synthesis (highpmf).
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DOI：
10.1016/0005-2728(76)90037-2
发表时间：
1976-09
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
J. Mills;A. Telfer;J. Barber
通讯作者：
J. Mills;A. Telfer;J. Barber
PSII 冷却诱导光抑制后恢复过程中循环电子流的刺激
影响因子：
4.9
作者：
Huang, Wei;Zhang, Shi-Bao;Cao, Kun-Fang
通讯作者：
Cao, Kun-Fang
DOI：
10.1016/0005-2728(88)90115-6
发表时间：
1988-06
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
U. Pick;M. Weiss
通讯作者：
U. Pick;M. Weiss
DOI：
--
发表时间：
2008
期刊：
--
影响因子：
--
作者：
U. Schreiber;C. Klughammer
通讯作者：
U. Schreiber;C. Klughammer
DOI：
10.1111/j.1365-313x.2008.03680.x
发表时间：
2009
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Atsushi Takabayashi;Noriko Ishikawa;T. Obayashi;S. Ishida;J. Obokata;T. Endo;F. Sato
通讯作者：
Atsushi Takabayashi;Noriko Ishikawa;T. Obayashi;S. Ishida;J. Obokata;T. Endo;F. Sato