Detection and relative quantification of proteins by surface enhanced Raman using isotopic labels.

Detection and relative quantification of proteins by surface enhanced Raman using isotopic labels.
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使用同位素标签对蛋白质的检测和相对定量。

DOI:
10.1021/ja800772p
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发表时间:
2008-07-30
影响因子:
15
通讯作者:
Davisson VJ
Davisson VJ
中科院分区:
化学1区
文献类型:
--
作者:
Deb SK;Davis B;Knudsen GM;Gudihal R;Ben-Amotz D;Davisson VJ

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Accurate quantification of protein content and composition has been achieved using isotope-edited surface enhanced resonance Raman spectroscopy. Synthesis of isotopomeric Rhodamine dye-linked bioconjugation reagents enabled direct labeling of surface lysines on a variety of proteins. When separated in polyacrylamide gels and stained with silver nanoparticles. The spectral signatures reflect the expected statistical distribution of isotopomeric labels on the labeled proteins in the gel matrix format without interference from protein features.
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