The GRP94 gene of Yesso scallop (Patinopecten yessoensis): Characterization and expression regulation in response to thermal and bacterial stresses
The GRP94 gene of Yesso scallop (Patinopecten yessoensis): Characterization and expression regulation in response to thermal and bacterial stresses
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Yesso 扇贝 (Patinopecten yessoensis) 的 GRP94 基因：响应热和细菌胁迫的特征和表达调控
DOI：
10.1016/j.fsi.2018.06.018
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发表时间：
2018-09
影响因子：
4.7
通讯作者：
Zhenmin Bao
中科院分区：
文献类型：
--
作者：
Shuyue Wang;Xu Li;Tingting Li;Huizhen Wang;Xiangchao Zhang;Jiarun Lou;Qiang Xing;Xiaoli Hu;Zhenmin Bao
属于HSP 90家族的94-kDa葡萄糖调节蛋白（GRP 94）是内质网（ER）伴侣。它在ER质量控制中起着关键作用，并被认为是调节先天性和适应性免疫的特异性免疫伴侣。本研究对虾夷扇贝（Patinopecten yessoensis）aGRP 94基因（PyGRP 94）进行了克隆和序列分析。PyGRP 94的蛋白质序列与脊椎动物同源物高度保守，其N端有一个信号序列，C端有一个ER修复信号序列，并含有一个HATP酶C结构域。表达分析表明，PyGRP 94基因在早期胚胎中的转录产物来源于母体，合子期表达始于D形幼虫。该基因也在除平滑肌外的几乎所有成体组织中表达，在血细胞中表达水平最高。此外，PyGRP 94在热休克、革兰氏阳性菌（藤黄微球菌）和革兰氏阴性菌（鳗弧菌）感染后均能被诱导表达，但在V.鳗挑战结果表明PyGRP 94参与了热应激反应，并可能在扇贝天然免疫防御中发挥重要作用。
The 94-kDa glucose-regulated protein (GRP94) belonging to the HSP90 family is an endoplasmic reticulum (ER) chaperone. It plays critical roles in ER quality control, and has been implicated as a specialized immune chaperone to regulate both innate and adaptive immunity. In this study, we identified and characterized aGRP94gene (PyGRP94) from Yesso scallop (Patinopecten yessoensis). The protein sequence of PyGRP94 is highly conserved with its homologs in vertebrates, with a signal sequence in N-terminal, an ER retrieval signal sequence in C-terminal and a HATPase_c domain. Expression analysis suggests thatPyGRP94transcripts in early embryos are maternally derived and the zygotic expression is started from D-shaped larvae. This gene is also expressed in almost all the adult tissues examined except smooth muscle, with the highest expression level in hemocytes. Besides,PyGRP94was demonstrated to be induced by heat shock and both Gram-positive (Micrococcus luteus) and Gram-negative (Vibrio anguillarum) bacterial infection, with much more dramatic changes being observed afterV. anguillarumchallenge. Our results suggest the involvement ofPyGRP94in response to thermal stress, and that it might play an important role in the innate immune defense of scallop.
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DOI：
10.1016/j.bbamcr.2011.10.013
发表时间：
2012-03
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Marzec M;Eletto D;Argon Y
通讯作者：
Argon Y
DOI：
10.1016/j.cbpb.2014.03.001
发表时间：
2014-05
期刊：
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
影响因子：
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作者：
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通讯作者：
Kexue Ma;Guangwen Chen;Changying Shi;F. Cheng;He Dou;C. Feng;Dezeng Liu
影响因子：
4
作者：
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通讯作者：
P. Csermely;T. Schnaider;Z. Prohászka;G. Nardai
影响因子：
3.7
作者：
Feng L;Yu Q;Li X;Ning X;Wang J;Zou J;Zhang L;Wang S;Hu J;Hu X;Bao Z
通讯作者：
Bao Z
影响因子：
4.7
作者：
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通讯作者：
Dinglong Yang;Qing Wang;Lizhu Chen;Yongliang Liu;Ruiwen Cao;Huifeng Wu;Fei Li;Chenglong Ji