Morphological and functional properties distinguish the substance P and gastrin-releasing peptide subsets of excitatory interneuron in the spinal cord dorsal horn.
Morphological and functional properties distinguish the substance P and gastrin-releasing peptide subsets of excitatory interneuron in the spinal cord dorsal horn.
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DOI：
10.1097/j.pain.0000000000001406
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发表时间：
2019-03
期刊：
影响因子：
7.4
通讯作者：
Todd AJ
中科院分区：
文献类型：
--
作者：
Dickie AC;Bell AM;Iwagaki N;Polgár E;Gutierrez-Mecinas M;Kelly R;Lyon H;Turnbull K;West SJ;Etlin A;Braz J;Watanabe M;Bennett DLH;Basbaum AI;Riddell JS;Todd AJ
补充数字内容可在文本中获得。通过神经肽表达鉴定的浅表背角兴奋性中间神经元种群在形态、电生理和药理学特性上存在差异。这对理解疼痛处理具有启示意义。兴奋性中间神经元占浅表背角神经元的大多数，但尽管它们被认为与疼痛和瘙痒有关，但关于它们的组织和功能的信息仍然有限。我们最近发现了2个由胃泌素释放肽（GRP）或P物质（SP）表达定义的兴奋性中间神经元群体。在这里，我们证明了这些细胞在形态、电生理和药理学特性上表现出主要的差异。基于它们的体树突形态和放电模式，我们提出SP细胞对应于放射状细胞，放射状细胞通常表现出延迟放电。相比之下，大多数GRP细胞表现为瞬态或单峰放电，其中许多可能与所谓的瞬态中枢细胞相对应。与SP细胞不同，GRP细胞很少有长时间的本体脊髓突起，这表明它们主要参与局部加工。两个种群对神经调节剂的反应也存在差异，大部分SP细胞对去甲肾上腺素和5-HT有反应，很少有GRP细胞；对μ-阿片激动剂DAMGO的反应则相反。尽管最近的一项研究表明GRP细胞受伤害感受器支配，并被有害刺激强烈激活，但我们发现很少有GRP细胞接受来自表达trpv1传入事件的直接突触输入，并且它们很少磷酸化细胞外信号调节激酶以响应有害刺激。这些发现表明SP和GRP细胞在处理体感觉信息方面存在差异。
Supplemental Digital Content is Available in the Text. Superficial dorsal horn excitatory interneuron populations, as identified by neuropeptide expression, differ in morphological, electrophysiological, and pharmacological properties. This has implications for understanding pain processing. Excitatory interneurons account for the majority of neurons in the superficial dorsal horn, but despite their presumed contribution to pain and itch, there is still limited information about their organisation and function. We recently identified 2 populations of excitatory interneuron defined by expression of gastrin-releasing peptide (GRP) or substance P (SP). Here, we demonstrate that these cells show major differences in their morphological, electrophysiological, and pharmacological properties. Based on their somatodendritic morphology and firing patterns, we propose that the SP cells correspond to radial cells, which generally show delayed firing. By contrast, most GRP cells show transient or single-spike firing, and many are likely to correspond to the so-called transient central cells. Unlike the SP cells, few of the GRP cells had long propriospinal projections, suggesting that they are involved primarily in local processing. The 2 populations also differed in responses to neuromodulators, with most SP cells, but few GRP cells, responding to noradrenaline and 5-HT; the converse was true for responses to the μ-opioid agonist DAMGO. Although a recent study suggested that GRP cells are innervated by nociceptors and are strongly activated by noxious stimuli, we found that very few GRP cells receive direct synaptic input from TRPV1-expressing afferents, and that they seldom phosphorylate extracellular signal–regulated kinases in response to noxious stimuli. These findings indicate that the SP and GRP cells differentially process somatosensory information.
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DOI：
10.1113/jp275240
发表时间：
2018-05-01
期刊：
The Journal of physiology
影响因子：
--
作者：
Balachandar A;Prescott SA
通讯作者：
Prescott SA
影响因子：
7.4
作者：
Cameron D;Polgár E;Gutierrez-Mecinas M;Gomez-Lima M;Watanabe M;Todd AJ
通讯作者：
Todd AJ
影响因子：
64.5
作者：
Duan B;Cheng L;Bourane S;Britz O;Padilla C;Garcia-Campmany L;Krashes M;Knowlton W;Velasquez T;Ren X;Ross S;Lowell BB;Wang Y;Goulding M;Ma Q
通讯作者：
Ma Q
影响因子：
16.2
作者：
Foster, Edmund;Wildner, Hendrik;Tudeau, Laetitia;Haueter, Sabine;Ralvenius, William T.;Jegen, Monika;Johannssen, Helge;Hoesli, Ladina;Haenraets, Karen;Ghanem, Alexander;Conzelmann, Karl-Klaus;Boesl, Michael;Zeilhofer, Hanns Ulrich
通讯作者：
Zeilhofer, Hanns Ulrich
影响因子：
48
作者：
Cai D;Cohen KB;Luo T;Lichtman JW;Sanes JR
通讯作者：
Sanes JR