Enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases.
Enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases.
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DOI：
10.1038/srep08716
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发表时间：
2015-03-03
影响因子：
4.6
通讯作者：
Kolkenbrock S
中科院分区：
文献类型：
--
作者：
Hamer SN;Cord-Landwehr S;Biarnés X;Planas A;Waegeman H;Moerschbacher BM;Kolkenbrock S
甲壳素和壳聚糖低聚物具有多种生物活性，在医药、化妆品和农业等领域具有潜在的应用价值。这些性质可能不仅取决于聚合和乙酰化的程度，而且还取决于乙酰化（PA）的特定模式，当通过化学水解产生低聚物时，所述乙酰化（PA）的特定模式无法控制。为了确定PA对生物活性的影响，需要足够量的限定的壳聚糖低聚物。具有特异性PA的壳聚糖低聚物可以通过几丁质低聚物的酶促脱乙酰化来产生，但多样性受到可获得的几丁质脱乙酰酶数量少的限制。我们已经生产了特定的壳聚糖低聚物，其通过组合使用两种不同的几丁质脱乙酰酶，即来自根瘤菌属GRH 2的使第一单元脱乙酰的NodB和来自霍乱弧菌的使第二单元从非还原端开始脱乙酰的COD，从非还原端开始在前两个单元脱乙酰。两种几丁质脱乙酰酶都接受彼此的产物，从而产生具有新的和确定的PA的壳聚糖低聚物。当扩展到进一步的甲壳素脱乙酰酶，这种方法有可能产生一个完全定义的架构，一个大范围的新的壳聚糖低聚物。
Chitin and chitosan oligomers have diverse biological activities with potentially valuable applications in fields like medicine, cosmetics, or agriculture. These properties may depend not only on the degrees of polymerization and acetylation, but also on a specific pattern of acetylation (PA) that cannot be controlled when the oligomers are produced by chemical hydrolysis. To determine the influence of the PA on the biological activities, defined chitosan oligomers in sufficient amounts are needed. Chitosan oligomers with specific PA can be produced by enzymatic deacetylation of chitin oligomers, but the diversity is limited by the low number of chitin deacetylases available. We have produced specific chitosan oligomers which are deacetylated at the first two units starting from the non-reducing end by the combined use of two different chitin deacetylases, namely NodB from Rhizobium sp. GRH2 that deacetylates the first unit and COD from Vibrio cholerae that deacetylates the second unit starting from the non-reducing end. Both chitin deacetylases accept the product of each other resulting in production of chitosan oligomers with a novel and defined PA. When extended to further chitin deacetylases, this approach has the potential to yield a large range of novel chitosan oligomers with a fully defined architecture.
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影响因子：
14.9
作者：
Rose, TM;Henikoff, JG;Henikoff, S
通讯作者：
Henikoff, S
影响因子：
3.1
作者：
Chang, KLB;Tsai, G;Fu, WR
通讯作者：
Fu, WR
影响因子：
3.1
作者：
Hamer, Stefanie Nicole;Moerschbacher, Bruno Maria;Kolkenbrock, Stephan
通讯作者：
Kolkenbrock, Stephan
影响因子：
5.4
作者：
Khoushab F;Yamabhai M
通讯作者：
Yamabhai M
DOI：
10.1073/pnas.76.9.4350
发表时间：
1979-01-01
影响因子：
11.1
作者：
TOWBIN, H;STAEHELIN, T;GORDON, J
通讯作者：
GORDON, J