Nkx2.2+ progenitors generate somatic motoneurons in the chick spinal cord.
Nkx2.2+ progenitors generate somatic motoneurons in the chick spinal cord.
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DOI：
10.1371/journal.pone.0051581
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ikenaka K
中科院分区：
文献类型：
--
作者：
Gotoh H;Ono K;Nomura T;Takebayashi H;Harada H;Nakamura H;Ikenaka K
神经元的异质类存在于脊髓中，并且对于其功能是必不可少的。神经前体细胞中转录因子的表达模式决定发育过程中的神经元亚型。Nkx2.2在位于Olig2阳性池腹侧的祖细胞池中表达，并且对于脊髓中的V3中间神经元生成以及对于后脑中的内脏运动神经元生成是必不可少的。然而，Nkx2.2阳性祖细胞是否产生不同类型的神经元尚未完全了解。使用鸡谱系追踪方法在遗传学定义的方式，我们发现，Nkx2.2表达祖细胞分化成一般内脏运动神经元以及SIM1阳性V3中间神经元。令人惊讶的是，我们进一步观察到表达Nkx2.2的祖细胞分化成体细胞运动神经元。我们的研究结果表明，不同类型的运动神经元的来源比之前在鸡脊髓中预期的更复杂。
Heterogeneous classes of neurons are present in the spinal cord and are essential for its function. Expression patterns of transcription factors in neural progenitor cells determine neuron subtypes during development. Nkx2.2 is expressed in the progenitor cell pool located just ventrally to the Olig2-positive pool and is indispensable for V3 interneuron generation in the spinal cord and also for visceral motoneuron generation in the hindbrain. However, whether Nkx2.2-positive progenitor cells generate diverse classes of neuron is not fully understood. Using a chick lineage tracing method in a genetically-defined manner, we found that Nkx2.2-expressing progenitor cells differentiate into general visceral motoneurons as well as sim1-positive V3 interneurons. Surprisingly, we further observed that Nkx2.2-expressing progenitors differentiate into somatic motoneuron. Our findings suggest that the different classes of motoneurons are derived from more complex sources than were previously expected in the chick spinal cord.
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