Multimodal photoacoustic ophthalmoscopy in mouse.
Multimodal photoacoustic ophthalmoscopy in mouse.
复制标题
DOI：
10.1002/jbio.201200061
复制
发表时间：
2013-06
影响因子：
2.8
通讯作者：
Zhang, Hao F.
中科院分区：
文献类型：
--
作者：
Song, Wei;Wei, Qing;Feng, Liang;Sarthy, Vijay;Jiao, Shuliang;Liu, Xiaorong;Zhang, Hao F.
关键词：
光声检眼镜（PAOM）是一种测量视网膜光吸收的新型成像技术。由于小鼠模型被广泛用于各种视网膜疾病，如果PAOM能够成像小鼠眼睛，则可以进一步增强其能力。然而，实现小鼠视网膜高质量成像的挑战来自于小得多的眼球尺寸。在这里，我们报告了一种优化的成像系统，该系统集成了PAOM，光谱域光学相干断层扫描（SD-OCT）和自动荧光扫描激光检眼镜（AF-SLO），用于小鼠眼睛。通过对光感受器中表达绿色荧光蛋白（GFP）的转基因Nrl-GFP小鼠进行成像，证明了其多模态能力。SD-OCT为PAOM和AF-SLO的光学对准提供指导，并与高深度分辨率视网膜横截面进行互补对比。PAOM可视化视网膜血管和视网膜色素上皮黑色素，AF-SLO测量视网膜光感受器中gfp的表达。通过组织学和共聚焦显微镜验证了体内成像结果。
Photoacoustic ophthalmoscopy (PAOM) is a novel imaging technology that measures optical absorption in the retina. The capability of PAOM can be further enhanced if it could image mouse eyes, because mouse models are widely used for various retinal diseases. The challenges in achieving high-quality imaging of mouse retina, however, come from the much smaller eyeball size. Here, we report an optimized imaging system, which integrates PAOM, spectral-domain optical coherence tomography (SD-OCT), and autofluorescence-scanning laser ophthalmoscopy (AF-SLO), for mouse eyes. Its multimodal capability was demonstrated by imaging transgenic Nrl-GFP mice that express green fluorescent protein (GFP) in photoreceptors. SD-OCT provided guidance of optical alignment for PAOM and AF-SLO, and complementary contrast with high depth-resolution retinal cross sections. PAOM visualized the retinal vasculature and retinal pigment epithelium melanin, and AF-SLO measured GFP-expressing in retinal photoreceptors. The in vivo imaging results were verified by histology and confocal microscopy.
登录
查看更多内容
影响因子：
30.8
作者：
Mears, AJ;Kondo, M;Swaroop, A
通讯作者：
Swaroop, A
影响因子：
1.8
作者：
Paques, M;Sirnonutti, M;Sahel, JA
通讯作者：
Sahel, JA
影响因子：
3.3
作者：
Sarthy, V.;Hoshi, H.;Dudley, V. J.
通讯作者：
Dudley, V. J.
DOI：
10.1073/pnas.0508214103
发表时间：
2006-03-07
影响因子：
11.1
作者：
Akimoto, M;Cheng, H;Swaroop, A
通讯作者：
Swaroop, A
影响因子：
35
作者：
通讯作者：
--