Influence of alternating current electrokinetic forces and torque on the elongation of immobilized DNA
Influence of alternating current electrokinetic forces and torque on the elongation of immobilized DNA
复制标题
交流电动力和扭矩对固定化 DNA 伸长的影响
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
M. Pepper
中科院分区：
文献类型：
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作者：
W. A. Germishuizen;P. Tosch;A. Middelberg;C. Wälti;A. Davies;R. Wirtz;M. Pepper
作者研究了不同大小的脱氧核糖核酸 (DNA) 分子在高频交流电场的影响下的伸长和方向，这些分子通过末端硫醇固定在金电极上。由于在施加交流电场期间作用在分子上的力，DNA分子从随机卷曲伸长成延伸构象并沿着电场线定向。在 100 kHz-1 MHz 频率范围内观察到伸长，场强为 0.06-1.0 MV/m。所有测试的 DNA 片段（无论大小）的最大伸长率均在 200 至 300 kHz 之间的频率下发现。作用在 DNA 分子中诱导偶极子上的扭矩，辅以与介电泳力方向相反的方向偏置力，为延伸过程提供了主要贡献。延伸长度限于相对电极之间距离的一半或 DNA 轮廓长度，以较短者为准。此外，作者表明，伸长 DNA 分子的标准化长度与 DNA 的轮廓长度无关。 (C) 2005 年美国物理研究所。
The authors investigate the elongation and orientation of different-sized deoxyribose nucleic acid (DNA) molecules, tethered onto gold electrodes via a terminal thiol, under the influence of high frequency ac electric fields. The DNA molecules are elongated from a random coil into an extended conformation and orientated along the electric field lines as a result of the forces acting on the molecules during the application of the ac electric fields. Elongation was observed in the frequency range 100 kHz-1 MHz, with field strengths of 0.06-1.0 MV/m. Maximum elongation for all DNA fragments tested, irrespective of size, was found for frequencies between 200 and 300 kHz. The torque acting on the induced dipole in the DNA molecules, complemented by a directional bias force, opposite in direction to the dielectrophoretic force, provides the main contribution to the elongation process. The length of elongation is limited to either half the distance between opposing electrodes or to the contour length of the DNA, whichever is shorter. Further, the authors show that the normalized length of the elongated DNA molecules is independent of the contour length of the DNA. (C) 2005 American Institute of Physics.
影响因子：
14.9
作者：
Ralf M. Zimmermann;Edward C. Cox
通讯作者：
Ralf M. Zimmermann;Edward C. Cox