Molecular analysis of hybrid dysgenesis-induced derivatives of a P-element allele at the vg locus
Molecular analysis of hybrid dysgenesis-induced derivatives of a P-element allele at the vg locus
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vg 基因座 P 元素等位基因杂交发育不全诱导衍生物的分子分析
DOI：
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发表时间：
1988
影响因子：
5.3
通讯作者：
J. Bell
中科院分区：
文献类型：
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作者：
Jim A. Williams;S. Pappu;J. Bell
二级和三级衍生物的P-元件插入等位基因在退化（Vg）基因座诱导的杂交发育不全。这些衍生物的特征在于Southern分析，并在四种情况下，通过DNA测序。所发现的改变是P元件内部缺失、插入片段和/或相邻vg区DNA的缺失、或P元件序列新插入到现有P元件插入片段中。本文观察到的二级插入到P元件序列中的相对高的频率是不寻常的，因为二级插入在其他非基因筛选中很少被回收。确定等位基因对vg表达的影响。结果与插入通过转录干扰破坏vg基因表达的模型一致。
Secondary and tertiary derivatives of a P-element insertion allele at the vestigial (vg) locus were induced by hybrid dysgenesis. The derivatives were characterized by Southern analyses and, in four cases, by DNA sequencing. The alterations found were P-element internal deletions, deletions of the insert and/or adjacent vg region DNA, or novel insertions of P-element sequences into existing P-element inserts. The relatively high frequency of secondary insertions into P-element sequences observed herein is unusual, since secondary insertions have seldom been recovered in other dysgenic screens. The effects of the alleles on vg expression were determined. The results are consistent with a model in which the insertions disrupt vg gene expression by transcriptional interference.
影响因子：
14.9
作者：
HOOPES, BC;MCCLURE, WR
通讯作者：
MCCLURE, WR