Insecticidal activity of avidin and streptavidin against four species of pest Lepidoptera
Insecticidal activity of avidin and streptavidin against four species of pest Lepidoptera
复制标题
抗生物素蛋白和链霉抗生物素蛋白对四种鳞翅目害虫的杀虫活性
DOI：
10.1046/j.1570-7458.2001.00757.x
复制
发表时间：
2001
影响因子：
1.9
通讯作者：
C. Lilley
中科院分区：
文献类型：
--
作者：
N. Markwick;J. Christeller;L.C. Dochterty;C. Lilley
两种生物素结合蛋白，亲和素和链霉亲和素，被发现对四种鳞翅目昆虫的幼虫具有杀虫作用-浅棕色苹果蛾，Epiphyas postvittana（步行者）（fam.卷叶蛾科（Tortricidae），绿色头卷叶蛾，Planotorocto（Dugdale）（fam.卷叶蛾科（Tortricidae）、褐头卷叶蛾（Ctenopseustis dangerana）（步行者）（fam.马铃薯块茎蛾Phthorimaea operculella（Zeller）（fam.麦蛾科）。死亡发生在所有物种，但有一个广泛的易感性。P. operculella幼虫是最敏感的，9天后的LC 50分别为2.3 μg ml-1的抗生物素蛋白和1.4 μg ml-1的链霉抗生物素蛋白。E. 21天后，后vittana幼虫对抗生物素蛋白的LC 50为43.4 μg ml-1，C. 45.7 μg ml−1的抗生物素蛋白。虽然在最高剂量的抗生物素蛋白下，八爪蟾出现显著死亡，但没有足够的剂量-死亡率反应来计算该物种的LC 50。对于所有种属，在接近剂量范围内死亡率曲线陡峭，随后出现死亡率不随剂量显著增加且未达到100%的平台期。死亡率显着影响的量的生物素在饮食上的父母一代已经饲养。在富含生物素的地方，后代的显著死亡率要低得多：在无生物素饮食中饲养五代的postvittana在14天后的LC 50为5.1 μg ml−1，而在一般目的饮食中饲养的幼虫则为76.7 μg ml−1。使用这些蛋白质赋予转基因植物抗虫性的影响进行了讨论。
Two biotin‐binding proteins, avidin and streptavidin, were found to be insecticidal to the larvae of four species of Lepidoptera – light brown apple moth, Epiphyas postvittana (Walker) (fam. Tortricidae), green‐headed leaf‐roller, Planotortrix octo (Dugdale) (fam. Tortricidae), brown‐headed leaf‐roller, Ctenopseustis obliquana (Walker) (fam. Tortricidae) and potato tuber moth, Phthorimaea operculella (Zeller) (fam. Gelechiidae). Mortality occurred in all species, but there was a wide range in susceptibility. P. operculella larvae were the most susceptible with an LC50 of respectively, 2.3 μg ml−1 for avidin and 1.4 μg ml−1 for streptavidin after 9 days. E. postvittana larvae had an LC50 of 43.4 μg ml−1 for avidin after 21 days, and C. obliquana larvae of 45.7 μg ml−1 for avidin after 28 days. Although significant mortality occurred in P.octo at the highest doses of avidin, there was no sufficient dose‐mortality response to calculate an LC50 for this species. For all species mortality curves were steep over a close range of doses followed by a plateau where mortality did not increase significantly with dose and did not reach 100%. Mortality was significantly affected by the amount of biotin in the diet on which the parental generation had been reared. Where this was rich in biotin, significant mortality of the offspring was much lower: larval offspring of a colony of E. postvittana, reared for five generations on a biotin‐free diet had an LC50 of 5.1 μg ml−1 after 14 days compared with 76.7 μg ml−1 for larvae from a colony reared on general purpose diet. The implications for use of these proteins to confer insect resistance on transgenic plants are discussed.
影响因子：
14.9
作者：
ARGARANA, CE;KUNTZ, ID;CANTOR, CR
通讯作者：
CANTOR, CR