Transcriptional cofactor Mask2 is required for YAP-induced cell growth and migration in bladder cancer cell.
Transcriptional cofactor Mask2 is required for YAP-induced cell growth and migration in bladder cancer cell.
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DOI：
10.7150/jca.16438
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发表时间：
2016
影响因子：
3.9
通讯作者：
Cai Z
中科院分区：
文献类型：
--
作者：
Dong L;Lin F;Wu W;Huang W;Cai Z
高度保守的河马信号通路是参与肿瘤发生发展的重要途径。以往的研究发现，河马蛋白途径的转录辅助激活因子YAP在许多临床膀胱癌组织中都有高表达。为了探讨YAP及其辅助因子Mask2在膀胱癌中的作用，我们在膀胱癌细胞中过表达YAP，发现YAP能够促进膀胱癌细胞的生长和迁移。此外，我们还提供了MASK2基因敲除能够抑制膀胱癌细胞生长和迁移的证据。此外，我们还证明了Mask2基因的敲除能够抑制过度YAP诱导的膀胱癌细胞的生长和迁移。为了解释YAP/Mask2复合体在膀胱癌中的作用，我们检测了参与细胞生长和迁移的Hippo信号通路的靶基因的表达，发现过表达的YAP能够上调靶基因的表达，而缺失Mask2可以下调靶基因的表达。综上所述，我们证明了Mask2在YAP诱导的膀胱癌细胞生长和迁移中是必需的，它是通过Hippo途径的靶基因实现的。
The highly conserved Hippo signaling pathway is an important pathway involved in tumorigenesis and development. In previous studies, YAP, the transcription coactivator of Hippo pathway, is found to be highly expressed in many clinical bladder cancer samples. To investigate the function of YAP and its cofactor Mask2 in bladder cancer, we overexpress YAP in bladder cancer cells and discover that YAP is able to promote bladder cancer cell growth and migration. In addition, we provide evidence that knockdown of Mask2 is able to repress bladder cancer cell growth and migration. Furthermore, we demonstrate knockdown of Mask2 is able to inhibit bladder cancer cell growth and migration induced by the excessive YAP. To explain the function of YAP/Mask2 complex in bladder cancer, we check the target genes' expression of Hippo signaling pathway involved in cell growth and migration and find overexpressed YAP is able to upregulate the target genes' expression while depletion of Mask2 downregulates them. Taken together, we demonstrate that Mask2 is required for the function of bladder cancer cell growth and migration induced by YAP via the target genes of Hippo pathway.
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影响因子：
9.2
作者：
Sansores-Garcia, Leticia;Atkins, Mardelle;Moya, Ivan M.;Shahmoradgoli, Maria;Tao, Chunyao;Mills, Gordon B.;Halder, Georg
通讯作者：
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影响因子：
5.4
作者：
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通讯作者：
Ayyavoo, V
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影响因子：
3.8
作者：
Liu JY;Li YH;Lin HX;Liao YJ;Mai SJ;Liu ZW;Zhang ZL;Jiang LJ;Zhang JX;Kung HF;Zeng YX;Zhou FJ;Xie D
通讯作者：
Xie D
影响因子：
8.4
作者：
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通讯作者：
Olalla Saad, Sara T.
DOI：
10.1016/j.bbadis.2006.07.010
发表时间：
2006-09-01
影响因子：
6.2
作者：
Traina, Fabiola;Favaro, Patricia M. B.;Saad, Sara T. O.
通讯作者：
Saad, Sara T. O.