The AplI Restriction-Modification System in an Edible Cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, Recognizes the Nucleotide Sequence 5′-CTGCAG-3′
The AplI Restriction-Modification System in an Edible Cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, Recognizes the Nucleotide Sequence 5′-CTGCAG-3′
复制标题
食用蓝藻、钝顶节旋藻（螺旋藻）NIES-39 中的 AplI 限制性修饰系统可识别核苷酸序列 5-CTGCAG-3
DOI：
10.1271/bbb.120919
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Y. Tabuse
中科院分区：
文献类型：
--
作者：
Hideaki Shiraishi;Y. Tabuse
限制性内切酶在钝顶节旋藻（Arthrospira platensis）中对外源DNA的降解是在这种具有经济价值的生物体中进行基因转移实验的潜在障碍。我们在大肠杆菌中过量生产了参与A. Platensis NIES-39。由推定的II型限制酶基因NIES39_K04640产生的蛋白质显示出内切核酸酶活性，其切割序列5 ′-CTGCAG-3 ′内第5位A和第6位G之间的DNA。我们将这种酶命名为AplI。来自相邻基因NIES39_K04650的蛋白质编码推定的DNA（胞嘧啶-5-）-甲基转移酶，通过修饰识别序列中第四位的C（而不是第一位的C）使DNA分子对AplI具有抗性。这种修饰酶，M.AplI，应该可以用于将DNA分子转化为抗AplI的形式，用于基因转移实验。本文还对节旋藻各种菌株的限制性内切酶进行了综述。
The degradation of foreign DNAs by restriction enzymes in an edible cyanobacterium, Arthrospira platensis, is a potential barrier for gene-transfer experiments in this economically valuable organism. We overproduced in Escherichia coli the proteins involved in a putative restriction-modification system of A. platensis NIES-39. The protein produced from the putative type II restriction enzyme gene NIES39_K04640 exhibited an endonuclease activity that cleaved DNA within the sequence 5'-CTGCAG-3' between the A at the fifth position and the G at the sixth position. We designated this enzyme AplI. The protein from the adjacent gene NIES39_K04650, which encodes a putative DNA (cytosine-5-)-methyltransferase, rendered DNA molecules resistant to AplI by modifying the C at the fourth position (but not the C at the first position) in the recognition sequence. This modification enzyme, M.AplI, should be useful for converting DNA molecules into AplI-resistant forms for use in gene-transfer experiments. A summary of restriction enzymes in various Arthrospira strains is also presented in this paper.
影响因子：
14.9
作者：
Sigrist CJ;Cerutti L;de Castro E;Langendijk-Genevaux PS;Bulliard V;Bairoch A;Hulo N
通讯作者：
Hulo N