Epstein-Barr virus infection is common in inflamed gastrointestinal mucosa.
Epstein-Barr virus infection is common in inflamed gastrointestinal mucosa.
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DOI：
10.1007/s10620-012-2116-5
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发表时间：
2012-07
影响因子：
3.1
通讯作者：
Gulley, Margaret L.
中科院分区：
文献类型：
--
作者：
Ryan, Julie L.;Shen, You-Jun;Morgan, Douglas R.;Thorne, Leigh B.;Kenney, Shannon C.;Dominguez, Ricardo L.;Gulley, Margaret L.
Epstein-Barr病毒（EBV）存在于10%的胃腺癌的恶性上皮细胞中，然而该病毒在正常胃肠道粘膜中的定位在很大程度上是未知的。在本研究中，我们在胃炎标本（n=89）、正常胃和结肠粘膜（n=14）、克罗恩病（n=9）和溃疡性结肠炎（n=11）组织中检测了EBV DNA和局部病毒基因产物。一系列敏感和特异性的定量聚合酶链反应针对EBV基因组的六个不同区域：BamH1W， EBNA1, LMP1, LMP2， BZLF1和EBER1。通过EBV编码RNA （EBER）原位杂交和病毒潜伏蛋白LMP1和LMP2以及病毒裂解蛋白BMRF1和BZLF1的免疫组织化学染色来定位EBV感染。CD20免疫染色检测B淋巴细胞。EBV DNA在正常胃粘膜中基本检测不到，但在46%的胃炎病变、44%的正常结肠粘膜、55%的克罗恩病和64%的溃疡性结肠炎样本中存在。EBV DNA水平超出了炎症组织中B淋巴细胞数量的预期，表明EBV优先定位于炎症性胃肠道病变。组织化学染色显示部分pcr阳性病变淋巴样细胞有EBER表达。通过定量PCR检测，两种溃疡性结肠炎组织淋巴样细胞中均表达了病毒裂解病毒蛋白BMRF1和BZLF1，两者的病毒载量均较高。感染eb病毒的淋巴细胞经常出现在发炎的胃和结肠粘膜中。一些病变中活跃的病毒复制增加了与病毒相关的胃肠道炎症持续存在的可能性。
Epstein-Barr virus (EBV) is present in the malignant epithelial cells of 10% of all gastric adenocarcinomas, however localization of the virus in normal gastrointestinal mucosa is largely unexplored. In the current study, we measured EBV DNA and localized viral gene products in gastritis specimens (n=89), normal gastric and colonic mucosa (n=14), Crohn’s disease (n=9), and ulcerative colitis (n=11) tissues. A battery of sensitive and specific quantitative polymerase chain reactions targeted six disparate regions of the EBV genome: BamH1W, EBNA1, LMP1, LMP2, BZLF1, and EBER1. EBV infection was localized by EBV-encoded RNA (EBER) in situ hybridization and by immunohistochemical stains for viral latent proteins LMP1 and LMP2 and for viral lytic proteins BMRF1 and BZLF1. B lymphocytes were identified using CD20 immunostains. EBV DNA was essentially undetectable in normal gastric mucosa but was present in 46% of gastritis lesions, 44% of normal colonic mucosa, 55% of Crohn’s disease, and 64% of ulcerative colitis samples. Levels of EBV DNA exceeded what would be expected based on the numbers of B lymphocytes in inflamed tissues, suggesting that EBV is preferentially localized to inflammatory gastrointestinal lesions. Histochemical staining revealed EBER expression in lymphoid cells of some PCR-positive lesions. The viral lytic viral proteins, BMRF1 and BZLF1, were expressed in lymphoid cells of two ulcerative colitis tissues, both of which had relatively high viral loads by quantitative PCR. EBV-infected lymphocytes are frequently present in inflamed gastric and colonic mucosa. Active viral replication in some lesions raises the possibility of virus-related perpetuation of gastrointestinal inflammation.
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DOI：
10.1097/00129039-200306000-00010
发表时间：
2003-06-01
影响因子：
1.6
作者：
Cho, HJ;Kim, JY;Lee, SS
通讯作者：
Lee, SS
影响因子：
11.8
作者：
Ling, PD;Vilchez, RA;Butel, JS
通讯作者：
Butel, JS
影响因子：
6.3
作者：
Kojima, Yasuyuki;Mogaki, Masatoshi;Nagahori, Kaoru
通讯作者：
Nagahori, Kaoru
影响因子：
3.1
作者：
Maher, Maha M.;Nassar, Mahmoud I.
通讯作者：
Nassar, Mahmoud I.
影响因子：
3.3
作者：
Gulley, ML;Pulitzer, DR;Schneider, BG
通讯作者：
Schneider, BG