Characterization of three pathway-specific regulators for high production of monensin in Streptomyces cinnamonensis
Characterization of three pathway-specific regulators for high production of monensin in Streptomyces cinnamonensis
复制标题
肉桂链霉菌高产莫能菌素的三种途径特异性调节因子的表征
DOI：
10.1007/s00253-017-8353-y
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发表时间：
2017-07
影响因子：
5
通讯作者：
Zhao Guang-Rong
中科院分区：
文献类型：
--
作者：
Tang Zheng-Kun;Li Xiao-Mei;Pang Ai-Ping;Lin Chun-Yan;Zhang Yue;Zhang Jie;Qiao Jianjun;Zhao Guang-Rong
莫能菌素是一种聚醚类离子载体抗生素，由肉桂链霉菌产生，在世界范围内被用作动物饲养领域的抗球虫剂和促生长剂。莫能菌素生物合成基因簇（mon）已被报道。在这项研究中，阐明了三个pupillin途径特异性调节剂（MonH，MonRI和MonRII）的潜在功能。基因失活、互补和过表达的结果表明，MonH、MonRI和MonRII正调控莫能菌素的产生。MonH和MonRI都是莫能菌素生物合成所必需的，而MonRII是非必需的，并且可以被monRI的额外表达完全取代。通过半定量逆转录聚合酶链反应（RT-PCR）和电泳迁移率变动分析（EMSA）的mon簇的转录分析揭示了共调节级联过程。MonH上调monRII的转录，而MonRII反过来又增强monRI的转录。MonRII是一种自动阻遏物，而MonRI是一种自动激活物。MonH激活monCII-monE的转录，并上调被MonRII抑制的monT的转录。monAX和monD被MonRI激活，并被MonRII上调。通过MonH、MonRI和MonRII对这些后聚酮合酶（post-PKS）基因的共调节将有助于莫能菌素的高产量。这些结果为链霉菌抗生素生物合成的转录调控级联提供了新的线索。
Monensin, a polyether ionophore antibiotic, is produced by Streptomyces cinnamonensis and worldwide used as a coccidiostat and growth-promoting agent in the field of animal feeding. The monensin biosynthetic gene cluster (mon) has been reported. In this study, the potential functions of three putatively pathway-specific regulators (MonH, MonRI, and MonRII) were clarified. The results from gene inactivation, complementation, and overexpression showed that MonH, MonRI, and MonRII positively regulate monensin production. Both MonH and MonRI are essential for monensin biosynthesis, while MonRII is non-essential and could be completely replaced by additional expression of monRI. Transcriptional analysis of the mon cluster by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and electrophoresis mobility shift assays (EMSAs) revealed a co-regulatory cascade process. MonH upregulates the transcription of monRII, and MonRII in turn enhances the transcription of monRI. MonRII is an autorepressor, while MonRI is an autoactivator. MonH activates the transcription of monCII-monE, and upregulates the transcription of monT that is repressed by MonRII. monAX and monD are activated by MonRI, and upregulated by MonRII. Co-regulation of those post-polyketide synthase (post-PKS) genes by MonH, MonRI, and MonRII would contribute to high production of monensin. These results shed new light on the transcriptional regulatory cascades of antibiotic biosynthesis in Streptomyces.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
2
作者：
Kim, Sang-Hun;Kim, Kwang-Youn;Ahn, Soon-Cheol
通讯作者：
Ahn, Soon-Cheol
影响因子：
5.7
作者：
Tumova, Lucie;Pombinho, Antonio R.;Korinek, Vladimir
通讯作者：
Korinek, Vladimir
影响因子：
--
作者：
Gallimore, AR;Stark, CBW;Spencer, JB
通讯作者：
Spencer, JB
通过糖基化中间体和终产物与响应调节剂 ChlF1 结合协调调节氯丝菌素生物合成
影响因子：
4.8
作者：
Li, Yue;Li, Jingjing;Tan, Huarong
通讯作者：
Tan, Huarong