Identification of the major expressed S-layer and cell surface-layer-related proteins in the model methanogenic archaea: Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A.
Identification of the major expressed S-layer and cell surface-layer-related proteins in the model methanogenic archaea: Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A.
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DOI：
10.1155/2012/873589
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Gunsalus RP
中科院分区：
文献类型：
--
作者：
Rohlin L;Leon DR;Kim U;Loo JA;Ogorzalek Loo RR;Gunsalus RP
许多古生菌的细胞膜含有由一种或多种表面暴露的蛋白质组成的蛋白质外壳或鞘。这些表层(S层)蛋白质有助于结构完整性，并保护脂膜免受环境挑战。为了探索甲烷球藻中这些层的物种多样性，利用蛋白质组学方法对巴氏甲烷链霉菌Fusaro菌株中主要的S层蛋白进行了鉴定。Mbar_A1758基因产物以多种形式存在，表观大小分别为130、120和100 kDa，与翻译后修饰一致，包括信号肽切除和蛋白质糖基化。在巴氏分支杆菌基因组中发现了一种与乙酰链霉菌C2a和玛氏甲烷链霉菌中的表层蛋白相关的蛋白质。这些数据揭示了甲烷肉毒科中独特的保守蛋白质特征和隐含的细胞表面结构，这在其他古生物中是不存在的。两种甲烷八叠球菌的同源基因表达谱显示，每个菌株都有一个S层拟同源基因的丰富表达。确定了各自的启动子元件，并证明它们在mRNA编码区和上游非翻译区是保守的。以前的食蟹猴基因组注释分配了80个基因与S层或表层相关的角色：然而，在所研究的68个基因中，没有一个是相对于实验确定的S层基因显著表达的。
Many archaeal cell envelopes contain a protein coat or sheath composed of one or more surface exposed proteins. These surface layer (S-layer) proteins contribute structural integrity and protect the lipid membrane from environmental challenges. To explore the species diversity of these layers in the Methanosarcinaceae, the major S-layer protein in Methanosarcina barkeri strain Fusaro was identified using proteomics. The Mbar_A1758 gene product was present in multiple forms with apparent sizes of 130, 120, and 100 kDa, consistent with post-translational modifications including signal peptide excision and protein glycosylation. A protein with features related to the surface layer proteins found in Methanosarcina acetivorans C2A and Methanosarcina mazei Goel was identified in the M. barkeri genome. These data reveal a distinct conserved protein signature with features and implied cell surface architecture in the Methanosarcinaceae that is absent in other archaea. Paralogous gene expression patterns in two Methanosarcina species revealed abundant expression of a single S-layer paralog in each strain. Respective promoter elements were identified and shown to be conserved in mRNA coding and upstream untranslated regions. Prior M. acetivorans genome annotations assigned S-layer or surface layer associated roles of eighty genes: however, of 68 examined none was significantly expressed relative to the experimentally determined S-layer gene.
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DOI：
10.1155/2010/608243
发表时间：
2010-07-07
期刊：
Archaea (Vancouver, B.C.)
影响因子：
--
作者：
Ellen AF;Zolghadr B;Driessen AM;Albers SV
通讯作者：
Albers SV
DOI：
10.1007/978-3-642-05062-6_9
发表时间：
2010-01-01
期刊：
PROKARYOTIC CELL WALL COMPOUNDS: STRUCTURE AND BIOCHEMISTRY
影响因子：
--
作者：
Rachel, Reinhard
通讯作者：
Rachel, Reinhard
DOI：
10.1073/pnas.87.23.9083
发表时间：
1990-12-01
影响因子：
11.1
作者：
SOWERS, KR;ROBERTSON, DE;ROBERTS, MF
通讯作者：
ROBERTS, MF
影响因子：
4.2
作者：
Rohlin L;Gunsalus RP
通讯作者：
Gunsalus RP
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.