Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae).
Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae).
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濒危龙脑香科12个多态性微卫星标记的分离与鉴定
DOI：
10.1002/ece3.7077
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发表时间：
2021-01
影响因子：
2.6
通讯作者：
Tang L
中科院分区：
文献类型：
--
作者：
Wang C;Ma X;Tang L
对分布于海南岛和越南北部的濒危树种--海南厚朴的微卫星标记进行了分离和鉴定。根据下一代测序数据开发了26个微卫星标记，并在ABI 3730xl DNA分析仪上进行了毛细管电泳法基因分型。在华南血吸虫群体中发现了12个多态标记。遗传多样性分析表明，每个座位的等位基因数在2~6个之间，观察杂合度和期望杂合度分别为0~0.755和0.259~0.779。以中国厚朴为试材，检测了引物的转移性。3个微卫星标记为多态，7个微卫星标记为多态。结果表明，南方红鱼和南方红鱼具有相似的遗传多样性水平。一个邻居加入了树状图，将所有个体聚为两大类群，其中一个主要由华南华支睾吸虫组成，另一个由两个亚群组成，分别对应于华南华支睾吸虫和华夏华支睾吸虫。这12个多态微卫星标记可用于研究华南厚朴及其近缘物种的遗传多样性、种群分化、交配系统和精细空间遗传结构，有利于这些濒危但珍贵的厚朴物种的保护和恢复。首次报道了濒危树种海南厚朴的12个多态微卫星标记。这些新开发的微卫星标记可用于研究南方红豆杉及其近缘物种的遗传多样性、种群结构和交配系统，有利于这些濒危珍稀物种的保护和恢复。
Microsatellite markers were isolated and characterized for Hopea hainanensis Merrill & Chun, an endangered tree species with scattered distribution in Hainan Island and northern Vietnam. Twenty‐six microsatellite markers were developed based on next‐generation sequencing data and were genotyped by capillary electrophoresis on an ABI 3730xl DNA Analyzer. Twelve markers were found to be polymorphic in H. hainanensis. GENODIVE analyses indicated that the number of alleles ranged from 2 to 6 per locus, and the observed and expected heterozygosity varied from 0 to 0.755 and from 0.259 to 0.779, respectively. Primer transferability was tested with Hopea chinensis Hand.‐Mazz. and Hopea reticulata Tardieu, in which 3 and 7 microsatellite markers were found to be polymorphic, separately. The results showed that H. reticulata and H. hainanensis had similar levels of genetic diversity. A neighbor joining dendrogram clustered all individuals into two major groups, one of which was exclusively constituted by H. hainanensis, while the other consisted of two subgroups, corresponding to H. reticulata and H. chinensis, respectively. The 12 polymorphic microsatellite markers could be applied to study genetic diversity, population differentiation, mating system, and fine‐scale spatial genetic structures of H. hainanensis as well as its close relatives, facilitating the conservation and restoration of these endangered but valuable Hopea species. We reported, for the first time, twelve polymorphic microsatellite markers for an endangered tree species Hopea hainanensis Merrill & Chun. These newly developed microsatellite markers could be applied to study genetic diversity, population structure, and mating system of H. hainanensis, as well as its close relatives, facilitating the conservation and restoration of these endangered and valuable Hopea species.
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DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
影响因子：
4.9
作者：
Finger, A.;Kettle, C. J.;Ghazoul, J.
通讯作者：
Ghazoul, J.
影响因子：
2.2
作者：
Ismail, S. A.;Ghazoul, J.;Kettle, C. J.
通讯作者：
Kettle, C. J.
影响因子：
3.6
作者：
Guo JJ;Shang SB;Wang CS;Zhao ZG;Zeng J
通讯作者：
Zeng J
影响因子：
2.2
作者：
Kalinowski, Steven T.;Taper, Mark L.
通讯作者：
Taper, Mark L.