Mutations in cell elongation genes mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells.
Mutations in cell elongation genes mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells.
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DOI：
10.1111/mmi.12148
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发表时间：
2013-03
影响因子：
3.6
通讯作者：
Niki H
中科院分区：
文献类型：
--
作者：
Shiomi D;Toyoda A;Aizu T;Ejima F;Fujiyama A;Shini T;Kohara Y;Niki H
RodZ与MreB相互作用，这两种因子都是维持大肠杆菌杆状所必需的。MreB组装成细丝调节一组合成肽聚糖（PG）层的酶的亚细胞排列。然而，MreB的聚合如何决定细菌细胞的杆状仍然是未知的。调节因子可能参与控制MreB的功能和动力学。我们分离出抑制突变，以部分恢复rodZ缺失突变体中的杆状，并发现一些抑制突变发生在mreB中。所有mreB突变都在MreB的IA结构域中或附近。这些mreB突变改变了体内MreB细丝的性质。此外，在PBP2和RodA的周质区发现了抑制突变，由mrdA和mrdB基因编码。与MreB和RodZ类似，PBP2和RodA对细胞壁延伸过程至关重要。因此，我们发现MreB的IA结构域和PBP2和RodA的周质结构域中的突变可以恢复ΔrodZ细胞的生长和杆状，这可能是通过改变该过程中MreB的需求。
RodZ interacts with MreB and both factors are required to maintain the rod shape of Escherichia coli. The assembly of MreB into filaments regulates the subcellular arrangement of a group of enzymes that synthesizes the peptidoglycan (PG) layer. However, it is still unknown how polymerization of MreB determines the rod shape of bacterial cells. Regulatory factor(s) are likely to be involved in controlling the function and dynamics of MreB. We isolated suppressor mutations to partially recover the rod shape in rodZ deletion mutants and found that some of the suppressor mutations occurred in mreB. All of the mreB mutations were in or in the vicinity of domain IA of MreB. Those mreB mutations changed the property of MreB filaments in vivo. In addition, suppressor mutations were found in the periplasmic regions in PBP2 and RodA, encoded by mrdA and mrdB genes. Similar to MreB and RodZ, PBP2 and RodA are pivotal to the cell wall elongation process. Thus, we found that mutations in domain IA of MreB and in the periplasmic domain of PBP2 and RodA can restore growth and rod shape to ΔrodZ cells, possibly by changing the requirements of MreB in the process.
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影响因子：
64.8
作者：
DEBOER, P;CROSSLEY, R;ROTHFIELD, L
通讯作者：
ROTHFIELD, L
影响因子：
3.6
作者：
Divakaruni, Arun V.;Baida, Cyril;Gober, James W.
通讯作者：
Gober, James W.
影响因子：
3.7
作者：
Liu J;Chen CY;Shiomi D;Niki H;Margolin W
通讯作者：
Margolin W
影响因子：
11.4
作者：
Kruse, T;Moller-Jensen, J;Gerdes, K
通讯作者：
Gerdes, K
影响因子：
2.7
作者：
FUJISAKI, S;HARA, H;NISHINO, T
通讯作者：
NISHINO, T