Components of field potentials evoked by white matter stimulation in isolated slices of primary visual cortex: spatial distributions and synaptic order.
Components of field potentials evoked by white matter stimulation in isolated slices of primary visual cortex: spatial distributions and synaptic order.
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初级视觉皮层孤立切片中白质刺激引起的场电位组成：空间分布和突触顺序。
DOI：
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发表时间：
1990
影响因子：
2.5
通讯作者：
Mriganka Sur
中科院分区：
文献类型：
--
作者：
Ronald B. Langdon;Mriganka Sur
1.我们记录了大鼠初级视皮层横切片细胞外场电位的空间分布。通过白色物质/层VI边界附近的电刺激诱发反应，并沿沿着与椎板正交并与刺激部位相交的径向轴从层I至V取样（“光束上”记录）。为了评估“水平”连接的活动，我们还记录了沿着平行于皮质层的轴的轮廓（“离束”记录），通常在第三层。沿着轴。总的来说，我们的目标是扩大了解的生理学和组织的新皮层电路，并提供一个基础，从不同的实验和实验者的数据比较时，新皮层场电位用于可塑性和药理学的研究。2.反应是高度具体的皮质层。我们区分了四种主要成分：两种群体尖峰（“S1”和“S2”）和两种较慢的波形（“W1”和“W2”）。后者似乎代表了电流在顶树突的颗粒层。成分W1是最早的慢成分，是一个突触驱动的场电位偶极，在第一层为正，在第二层为负。基于对电流源密度（CSD）的估计，我们将其归因于去极化电流进入树突和/或细胞胞体的第二层，上升的树突内电流，和被动去极化的无活性树突膜的第一层。分量W1在刺激后的20 ms期间上升，并且在此后的50-100 ms期间福尔斯下降。组分W2在层I中也为正，但在层III中为最大负。它在刺激后上升约100 ms，并在随后的400-800 ms期间衰减。组分S1不依赖于突触传递，因为它在谷氨酸受体拮抗剂或低Ca 2+介质的应用期间持续存在。该分量在层III中最大，径向于刺激部位。在那里，它是一个负偏转，通常1-2 mV的幅度和持续约2 ms，与2和4.5 ms之间的潜伏期达到峰值。组件S1是最有可能的人口尖峰，由于同步发射的细胞胞体激活逆向通过无髓鞘传出轴突。4.成分S2是一个短的（小于20毫秒）突发的人口尖峰，特别是在第三层。单个S2尖峰非常类似于S1尖峰，我们建议，相同的神经元群体产生两者。然而，S2尖峰需要突触能传递。（400字处截断摘要）
1. We have recorded profiles of the spatial distributions of extracellular field potentials in transverse slices of rat primary visual cortex. Responses were evoked by electrical stimulation near the white matter/layer VI border and sampled from layers I to V along the radial axis orthogonal to the laminae and intersecting the stimulation site ("on-beam" recording). To assess the activity of "horizontal" connections, we also recorded profiles along axes parallel to the cortical lamination ("off-beam" recording), usually in layer III. Overall, our goal was to extend understanding of the physiology and organization of neocortical circuitry and to provide a basis for comparisons of data from different experiments and experimenters when neocortical field potentials are used in studies of plasticity and pharmacology. 2. Responses were highly specific with respect to the cortical layers. We distinguish four major components: two kinds of population spike ("S1" and "S2") and two slower waveforms ("W1" and "W2"). The latter appear to represent flow of current in apical dendrites of the supragranular layers. Component W1, the earliest slow component, is a synaptically driven field potential dipole that is positive in layer I and negative in layer II. Based on estimates of current source densities (CSDs), we attribute this to entry of depolarizing current into dendrites and/or cell somata in layer II, ascending intradendritic current, and passive depolarization of inactive dendritic membrane in layer I. Component W1 rises during the 20 ms after stimulation and falls during the 50-100 ms thereafter. Component W2 is also positive in layer I but maximally negative in layer III. It rises for approximately 100 ms after stimulation and decays during the following 400-800 ms. 3. Component S1 does not depend on synaptic transmission because it persists during the application of glutamate receptor antagonists or medium that is low in Ca2+. This component is largest in layer III, radial to the site of stimulation. There, it is a negative deflection, typically 1-2 mV in amplitude and lasting roughly 2 ms, with a latency to peak between 2 and 4.5 ms. Component S1 is most likely a population spike due to synchronized firing of cell somata activated antidromically via unmyelinated efferent axons. 4. Component S2 is a short (less than 20 ms) burst of population spikes specifically in layer III. Individual S2 spikes closely resemble S1 spikes, and we propose that the same neuronal population generates both. However, S2 spikes require glutamatergic synaptic transmission.(ABSTRACT TRUNCATED AT 400 WORDS)
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影响因子：
2.5
作者：
MCCORMICK, DA;CONNORS, BW;PRINCE, DA
通讯作者：
PRINCE, DA
影响因子：
33.6
作者：
通讯作者：
--
体外大鼠新皮质神经元中的 EPSP。
影响因子：
2.5
作者：
Sutor,B;Hablitz,JJ
通讯作者：
Hablitz,JJ
影响因子：
2.5
作者：
CHAGNACAMITAI, Y;CONNORS, BW
通讯作者：
CONNORS, BW
影响因子：
2.5
作者：
CHAGNACAMITAI, Y;CONNORS, BW
通讯作者：
CONNORS, BW