Sihler's whole mount nerve staining technique: a review.
Sihler's whole mount nerve staining technique: a review.
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Sihler 的整体神经染色技术：回顾。
DOI：
10.3109/10520290903048384
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发表时间：
2010-02
影响因子：
1.6
通讯作者：
Sanders, I.
中科院分区：
文献类型：
--
作者：
Mu, L.;Sanders, I.
关键词：
Sihler氏染色是一种全支架神经染色技术，在染色神经的同时使其他软组织半透明或透明。在将标本固定在中和福尔马林中、在氢氧化钾中浸软、在乙酸中脱钙、在埃利希苏木精中染色、在乙酸中脱色并在甘油中澄清后，它允许绘制器官、骨骼肌、粘膜、皮肤和其他结构的整个神经供应模式。与其他解剖学方法相比，Sihler染色的独特优势在于染色标本内的所有神经都可以在其三维位置中可视化。迄今为止，Sihler染色是显示骨骼肌精确肌内分支和分布模式的最佳工具，这不仅对解剖学家，而且对生理学家和临床医生都很重要。哺乳动物骨骼肌内神经结构的先进知识对于了解肌肉功能、进行电生理实验和开发新型神经外科技术至关重要。本文对Sihler染色法进行了详细介绍，并对其在神经标测中的应用进行了综述。特别强调染色程序和故障排除，优势和局限性，应用，对神经科学的主要贡献，生理和临床意义，以及值得未来研究的进一步技术改进领域。
Sihler's stain is a whole mount nerve staining technique that renders other soft tissue translucent or transparent while staining the nerves. It permits mapping of entire nerve supply patterns of organs, skeletal muscles, mucosa, skin, and other structures after the specimens are fixed in neutralized formalin, macerated in potassium hydroxide, decalcified in acetic acid, stained in Ehrlich's hematoxylin, destained in acetic acid, and cleared in glycerin. The unique advantage of Sihler's stain over other anatomical methods is that all the nerves within the stained specimen can be visualized in their three-dimensional positions. To date, Sihler's stain is the best tool for demonstrating the precise intramuscular branching and distribution patterns of skeletal muscles, which are important not only for anatomists, but also for physiologists and clinicians. Advanced knowledge of the neural structures within mammalian skeletal muscles is critical for understanding muscle functions, performing electrophysiological experiments and developing novel neurosurgical techniques. In this review, Sihler's stain is described in detail and its use in nerve mapping is surveyed. Special emphasis is placed on staining procedures and troubleshooting, strengths and limitations, applications, major contributions to neuroscience, physiological and clinical significance, and areas for further technical improvement that deserve future research.
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