Intracellular recording of action potentials by nanopillar electroporation.
Intracellular recording of action potentials by nanopillar electroporation.
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DOI：
10.1038/nnano.2012.8
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发表时间：
2012-02-12
影响因子：
38.3
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中科院分区：
文献类型：
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动作电位在神经系统和许多细胞过程中起着中心作用，特别是那些涉及离子通道的过程。动作电位的精确测量需要细胞膜和测量电极之间的有效耦合。细胞内记录方法，如膜片夹紧，包括通过电极进入细胞内部，测量细胞膜上的电压或电流，允许动作电位的幅度和形状以高信噪比忠实地记录下来。然而，细胞内方法的侵入性通常将记录时间限制在几个小时内，并且它们的复杂性使得同时记录多个细胞变得困难。细胞外记录方法，如多电极阵列和多晶体管阵列，是非侵入性的，允许长期和多路测量。然而，细胞外记录牺牲了细胞和电极之间的一对一对应关系，并且还受到显著降低的信号强度和质量的影响。因此，细胞外技术不能准确地记录动作电位，以探索离子通道的特性。因此，离子通道药物的药理学筛选通常通过低通量细胞内记录方法进行。使用纳米线晶体管、纳米管耦合晶体管和微金脊及相关电极，可以显著提高记录动作电位的信号强度。在这里，我们发现垂直纳米柱电极可以长时间记录培养心肌细胞的细胞外和细胞内动作电位，信号强度和质量都很好。此外，通过纳米级电穿孔和再密封过程，可以在细胞外和细胞内记录之间反复切换。此外，垂直纳米柱电极可以检测到靶向离子通道的药物引起的动作电位的细微变化。
Action potentials have a central role in the nervous system and in many cellular processes, notably those involving ion channels. The accurate measurement of action potentials requires efficient coupling between the cell membrane and the measuring electrodes. Intracellular recording methods such as patch clamping involve measuring the voltage or current across the cell membrane by accessing the cell interior with an electrode, allowing both the amplitude and shape of the action potentials to be recorded faithfully with high signal-to-noise ratios. However, the invasive nature of intracellular methods usually limits the recording time to a few hours, and their complexity makes it difficult to simultaneously record more than a few cells. Extracellular recording methods, such as multielectrode arrays and multitransistor arrays, are non-invasive and allow long-term and multiplexed measurements. However, extracellular recording sacrifices the one-to-one correspondence between the cells and electrodes, and also suffers from significantly reduced signal strength and quality. Extracellular techniques are not, therefore, able to record action potentials with the accuracy needed to explore the properties of ion channels. As a result, the pharmacological screening of ion-channel drugs is usually performed by low-throughput intracellular recording methods. The use of nanowire transistors,,, nanotube-coupled transistors and micro gold-spine and related electrodes,,,can significantly improve the signal strength of recorded action potentials. Here, we show that vertical nanopillar electrodes can record both the extracellular and intracellular action potentials of cultured cardiomyocytes over a long period of time with excellent signal strength and quality. Moreover, it is possible to repeatedly switch between extracellular and intracellular recording by nanoscale electroporation and resealing processes. Furthermore, vertical nanopillar electrodes can detect subtle changes in action potentials induced by drugs that target ion channels.
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影响因子：
--
作者：
Choi, D. S.;Fung, A. O.;Leung, M.
通讯作者：
Leung, M.
影响因子：
11.4
作者：
NEUMANN, E;SCHAEFERRIDDER, M;HOFSCHNEIDER, PH
通讯作者：
HOFSCHNEIDER, PH
影响因子：
10.8
作者：
Xie C;Hanson L;Xie W;Lin Z;Cui B;Cui Y
通讯作者：
Cui Y
DOI：
10.1073/pnas.1015589108
发表时间：
2011-03-08
影响因子：
11.1
作者：
Xie, Chong;Hanson, Lindsey;Cui, Bianxiao
通讯作者：
Cui, Bianxiao
影响因子：
10.8
作者：
Timko BP;Cohen-Karni T;Yu G;Qing Q;Tian B;Lieber CM
通讯作者：
Lieber CM