Activin A as a novel biomarker for colorectal adenocarcinoma in humans.
Activin A as a novel biomarker for colorectal adenocarcinoma in humans.
复制标题
激活素 A 作为人类结直肠腺癌的新型生物标志物。
DOI：
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复制
发表时间：
2015
影响因子：
3.3
通讯作者：
Z
中科院分区：
文献类型：
--
作者：
W. S.;Q. Y.;Limin Niu;D. Xie;Xueling Cui;Z
目的
结肠直肠癌的早期诊断因缺乏可靠的血清生物标志物而变得复杂。本研究旨在探讨血清激活素A水平是否可用于诊断这种疾病。
患者和方法
在这项研究中，我们测量了结直肠腺癌，良性结直肠息肉患者以及健康受试者的激活素A水平。
结果
我们发现，大肠腺癌患者的激活素A水平显著高于息肉患者和健康对照组。此外，激活素A水平与结直肠癌的分期密切相关。术后患者的激活素A水平显著降低。免疫组织化学染色表明，高水平的激活素A存在于腺癌组织样品中，但不存在于非癌样品中。RT-PCR进一步证实激活素A的β A亚基mRNA在大多数癌组织中显著过表达。Western印迹结果进一步证明了癌症样品中激活素A水平的升高。
结论
总的来说，这些发现表明结直肠腺癌直接将激活素A分泌到血流中。检测血清激活素A水平可作为临床诊断和筛查的可靠工具。
OBJECTIVE
Early diagnostics of colorectal cancer is complicated by the lack of reliable serum biomarkers. This study aimed to investigate if the serum level of activin A can be used for diagnostics of this disease.
PATIENTS AND METHODS
In this study we measured the level of activin A in patients with colorectal adenocarcinoma, benign colorectal polyps, as well as in healthy subjects.
RESULTS
We found that the level of activin A was significantly higher in patients with colorectal adenocarcinoma, as compared to patients with polyps and healthy controls. Furthermore, activin A levels correlated well with the stage of colorectal cancer. The level of activin A was substantially reduced in post-operative patients. Immunohistochemical staining demonstrated that high levels of activin A were present in the adenocarcinoma tissue samples but not in the non-cancerous samples. RT-PCR further confirmed that mRNA of ßA subunit of activin A is significantly over-expressed in the majority of cancerous samples. Western blotting results further demonstrated the elevated level of activin A in cancer samples.
CONCLUSIONS
Taken together, the findings suggest that colorectal adenocarcinomas directly secret activin A into the blood stream. Measuring the serum level of activin A might be used as a reliable diagnostic and screening tool in clinical practice.
DOI：
10.1016/j.bbrc.2009.02.029
发表时间：
2009-04-03
影响因子：
3.1
作者：
Licona-Limon, Paula;Aleman-Muench, German;Chimal-Monroy, Jesus;Macias-Silvac, Marina;Garcia-Zepeda, Eduardo A.;Matzuk, Martin M.;Fortoul, Teresa I.;Soldevila, Gloria
通讯作者：
Soldevila, Gloria
影响因子：
5.8
作者：
Islam, Md Soriful;Catherino, William H.;Ciarmela, Pasquapina
通讯作者：
Ciarmela, Pasquapina
影响因子：
158.5
作者：
MANDEL, JS;BOND, JH;EDERER, F
通讯作者：
EDERER, F