Measuring Breathing Patterns in Mice Using Whole-body Plethysmography.
Measuring Breathing Patterns in Mice Using Whole-body Plethysmography.
复制标题
使用全身体积描记法测量小鼠的呼吸模式。
作者：
Patricia Prada;Andrea Urpi;Laura Sánchez;P. Bianchi;Albert Quintana
呼吸功能障碍是全世界严重和致命疾病的主要原因之一。使用有效的实验模型和方法来研究肺部病理生理学对于预防、控制和治疗这些疾病是必要的。体积描记法是一种评估肺功能的技术，已广泛应用于小鼠的呼吸生理学表征。然而，经典的体积描记法存在技术限制，例如使用麻醉和动物固定。全身体积描记法（WBP）避免了这些问题，为评估有意识的动物的呼吸功能提供了一种非侵入性方法。 WBP 依赖于记录放置在密闭室内的动物的自主呼吸活动所产生的压力变化。在正常呼吸期间，压力变化与动物的呼吸模式成正比，从而可以测量呼吸频率和潮气量。这些参数通常用于评估不同生理和疾病模型中的肺功能。与经典的体积描记法相比，WBP 技术可以进行可重复的连续测量，因为它避免了动物束缚或使用麻醉。这些关键特征使 WBP 成为纵向研究的合适方法，可以评估生理和病理条件下进行性呼吸变化。该协议描述了使用 WBP 方法、数据分析和结果解释测量小鼠呼吸模式的程序。
Respiratory dysfunction is among the main cause of severe and fatal pathologies worldwide. The use of effective experimental models and methodologies for the study of the pulmonary pathophysiology is necessary to prevent, control and cure these diseases. Plethysmography, a technique for the assessment of lung function, has been widely applied in mice for the characterization of respiratory physiology. However, classical plethysmography methods present technical limitations such as the use of anesthesia and animal immobilization. Whole-body plethysmography (WBP) avoids these issues providing a non-invasive approach for the assessment of the respiratory function in conscious animals. WBP relies on the recording of pressure changes that are produced by the spontaneous breathing activity of an animal placed inside an airtight chamber. During normal respiration, pressure variation is directly proportional to the respiratory pattern of the animal allowing the measurement of the respiratory rate and tidal volume. These parameters are commonly used to evaluate pulmonary function in different physiological and disease models. In contrast to classical plethysmography methods, WBP technique allows reproducible serial measurements as it avoids animal restraint or the use of anesthesia. These key features rend WBP a suitable approach for longitudinal studies allowing the assessment of progressive respiratory alterations in physiological and pathological conditions. This protocol describes the procedures for the measurement of the breathing patterns in mice using the WBP method, the data analysis and results interpretation.
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影响因子：
3.3
作者：
Lundblad, LKA;Irvin, CG;Bates, JHT
通讯作者：
Bates, JHT
通过详细检查呼吸功能来评估肺部病理学。
DOI：
10.2353/ajpath.2010.100053
发表时间：
2010
期刊：
The American journal of pathology
影响因子：
--
作者：
Vaickus,LouisJ;Bouchard,Jacqueline;Kim,Jiyoun;Natarajan,Sudha;Remick,DanielG
通讯作者：
Remick,DanielG
影响因子：
6.1
作者：
Flanagan, Thomas W.;Sebastian, Melaine N.;Nichols, Charles D.
通讯作者：
Nichols, Charles D.
影响因子：
3.3
作者：
Adler, A;Cieslewicz, G;Irvin, CG
通讯作者：
Irvin, CG