Antibacterial Mechanism of Gloverin2 from Silkworm, Bombyx mori.
Antibacterial Mechanism of Gloverin2 from Silkworm, Bombyx mori.
复制标题
家蚕 Gloverin2 的抗菌机制。
DOI：
10.3390/ijms19082275
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发表时间：
2018-08-03
影响因子：
5.6
通讯作者：
Zhao P
中科院分区：
文献类型：
--
作者：
Wang Q;Guo P;Wang Z;Liu H;Zhang Y;Jiang S;Han W;Xia Q;Zhao P
Gloverin是一种富含甘氨酸的抗菌肽，仅存在于鳞翅目昆虫中。它通常通过昆虫的先天免疫系统被激活。本研究利用原核表达系统合成了家蚕Gloverin2重组蛋白（BmGlv2）。圆二色光谱分析表明，重组BmGlv2具有随机线圈结构，在15 ~ 82.5℃温度范围内相对稳定。抗菌活性分析表明，BmGlv2通过破坏细胞完整性，显著抑制革兰氏阴性菌大肠杆菌JM109和恶臭假单胞菌的生长。Western blotting和免疫荧光分析表明，BmGlv2在孵育后被细胞表面吸收，这可能是抗菌过程的第一步。我们的研究结果还证明了细胞壁成分脂多糖（LPS）诱导BmGlv2的构象从随机线圈转变为α-螺旋。随后，α-螺旋BmGlv2会招募更多的BmGlv2，形成更高的聚集状态。总的来说，这些发现扩大了我们对BmGlv2抗菌机制的理解。
Gloverin is one of the glycine-rich antimicrobial peptide exclusively found in Lepidoptera insects. It is generally activated through the innate immune system in insects. In this study, recombinant Gloverin2 from Bombyx mori (BmGlv2) was synthesized using a prokaryotic expression system. Circular dichroism spectroscopy showed that the recombinant BmGlv2 has random coil structure, which is relatively stable at the temperatures ranging from 15 to 82.5 °C. Antimicrobial activity analysis revealed that BmGlv2 significantly inhibited the growth of gram-negative bacteria, Escherichia coli JM109 and Pseudomonas putida, by disrupting cell integrity. Western blotting and immunofluorescence analyses suggested that BmGlv2 absorbed on the cell surface after incubation, which might be the first step in the antibacterial process. Our results also proved that the cell wall component lipopolysaccharides (LPS) induce a conformational change in BmGlv2 from a random coil to α-helix. Subsequently, α-helical BmGlv2 would recruit more BmGlv2 and form higher aggregation state. Collectively, these findings expand our understanding of antibacterial mechanism of BmGlv2.
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影响因子：
3.4
作者：
Moreno-Habel DA;Biglang-awa IM;Dulce A;Luu DD;Garcia P;Weers PM;Haas-Stapleton EJ
通讯作者：
Haas-Stapleton EJ
影响因子：
2.9
作者：
MANAVALAN, P;JOHNSON, WC
通讯作者：
JOHNSON, WC
影响因子：
2.9
作者：
HENNESSEY, JP;JOHNSON, WC
通讯作者：
JOHNSON, WC
影响因子：
5.5
作者：
Rittig, MG;Kaufmann, A;Dornand, J
通讯作者：
Dornand, J
DOI：
10.1111/j.1432-1033.1997.00614.x
发表时间：
1997-07-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Axen, A;Carlsson, A;Bennich, H
通讯作者：
Bennich, H