Genetically encoded intrabodies as high-precision tools to visualize and manipulate neuronal function.
Genetically encoded intrabodies as high-precision tools to visualize and manipulate neuronal function.
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DOI：
10.1016/j.semcdb.2021.11.004
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发表时间：
2022-06
影响因子：
7.3
通讯作者：
Trimmer, James S.
中科院分区：
文献类型：
--
作者：
Trimmer, James S.
基础神经科学研究采用多种形式的抗体作为不同应用中的关键试剂。虽然抗体的主要用途是作为免疫标记试剂，但神经科学家越来越多地使用细胞内抗体或胞内抗体。胞内抗体是基因编码用于在神经元内表达的重组抗体。这些可以用于靶向各种货物（荧光蛋白，报告基因，酶等）。到特定的分子和亚细胞域，以高精度报告和操纵神经元功能。胞内抗体具有所有遗传编码的重组抗体固有的优点，但代表不同的亚类，因为它们的结构允许它们在细胞内表达和功能。由于脑神经元具有巨大的分子、细胞和形态学复杂性，将其表达导向特定细胞类型的能力所提供的高精度，以及胞内抗体与这些细胞内靶标的选择性结合，使胞内抗体为神经科学研究提供了独特的优势。在神经元内表达的胞内抗体已用于基础神经科学研究中的多种目的。在这里，我提供了一个一般的背景，胞内抗体和他们的发展，和他们的新兴效用作为有价值的基础神经科学研究工具的例子。
Basic neuroscience research employs numerous forms of antibodies as key reagents in diverse applications. While the predominant use of antibodies is as immunolabeling reagents, neuroscientists are making increased use of intracellular antibodies or intrabodies. Intrabodies are recombinant antibodies genetically encoded for expression within neurons. These can be used to target various cargo (fluorescent proteins, reporters, enzymes, etc.) to specific molecules and subcellular domains to report on and manipulate neuronal function with high precision. Intrabodies have the advantages inherent in all genetically encoded recombinant antibodies but represent a distinct subclass in that their structure allows for their expression and function within cells. The high precision afforded by the ability to direct their expression to specific cell types, and the selective binding of intrabodies to targets within these allows intrabodies to offer unique advantages for neuroscience research, given the tremendous molecular, cellular and morphological complexity of brain neurons. Intrabodies expressed within neurons have been used for a variety of purposes in basic neuroscience research. Here I provide a general background to intrabodies and their development, and examples of their emerging utility as valuable basic neuroscience research tools.
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