Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s.
Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s.
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DOI：
10.3390/v12090914
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发表时间：
2020-08-20
期刊：
影响因子：
--
通讯作者：
Ito N
中科院分区：
文献类型：
--
作者：
Takahashi T;Inukai M;Sasaki M;Potratz M;Jarusombuti S;Fujii Y;Nishiyama S;Finke S;Yamada K;Sakai H;Sawa H;Nishizono A;Sugiyama M;Ito N
1957年在日本根除狂犬病之前的20世纪40年代，从东京的一只狗身上分离出的狂犬病毒小松川（Koma）是日本唯一存在的野外毒株（街头毒株）。虽然该毒株可能为研究狂犬病发病机制提供有用的模型，但对其遗传和表型特性知之甚少。值得注意的是，该菌株在分离后在啮齿动物中进行了连续传代，这表明它可能已经失去了作为街头菌株的生物学特性。在这项研究中，为了评估Koma菌株在研究狂犬病发病机制中的效用，我们检测了其遗传特性和体外和体内表型。全基因组遗传分析表明，与先前部分序列分析的结果一致，科马菌株与北极相关系统发育分支中的俄罗斯街头菌株密切相关。体外表型检测显示，Koma菌株和具有代表性的街头菌株的嗜神经性低于实验室菌株。用小鼠模型检查表明，科马毒株和街头毒株比实验室毒株更具有神经侵入性。这些发现表明，科马毒株保留了与街头毒株相似的表型，因此有助于研究狂犬病的发病机制。
The rabies virus strain Komatsugawa (Koma), which was isolated from a dog in Tokyo in the 1940s before eradication of rabies in Japan in 1957, is known as the only existent Japanese field strain (street strain). Although this strain potentially provides a useful model to study rabies pathogenesis, little is known about its genetic and phenotypic properties. Notably, this strain underwent serial passages in rodents after isolation, indicating the possibility that it may have lost biological characteristics as a street strain. In this study, to evaluate the utility of the Koma strain for studying rabies pathogenesis, we examined the genetic properties and in vitro and in vivo phenotypes. Genome-wide genetic analyses showed that, consistent with previous findings from partial sequence analyses, the Koma strain is closely related to a Russian street strain within the Arctic-related phylogenetic clade. Phenotypic examinations in vitro revealed that the Koma strain and the representative street strains are less neurotropic than the laboratory strains. Examination by using a mouse model demonstrated that the Koma strain and the street strains are more neuroinvasive than the laboratory strains. These findings indicate that the Koma strain retains phenotypes similar to those of street strains, and is therefore useful for studying rabies pathogenesis.
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影响因子：
3.8
作者：
Hampson K;Coudeville L;Lembo T;Sambo M;Kieffer A;Attlan M;Barrat J;Blanton JD;Briggs DJ;Cleaveland S;Costa P;Freuling CM;Hiby E;Knopf L;Leanes F;Meslin FX;Metlin A;Miranda ME;Müller T;Nel LH;Recuenco S;Rupprecht CE;Schumacher C;Taylor L;Vigilato MA;Zinsstag J;Dushoff J;Global Alliance for Rabies Control Partners for Rabies Prevention
通讯作者：
Global Alliance for Rabies Control Partners for Rabies Prevention
影响因子：
5.4
作者：
Brzózka, K;Finke, S;Conzelmann, KK
通讯作者：
Conzelmann, KK
DOI：
10.1292/jvms.17-0028
发表时间：
2017-06-10
期刊：
The Journal of veterinary medical science
影响因子：
--
作者：
Kimitsuki K;Yamada K;Shiwa N;Inoue S;Nishizono A;Park CH
通讯作者：
Park CH
DOI：
10.1073/pnas.0407289101
发表时间：
2004-11-16
影响因子：
11.1
作者：
Faber, M;Pulmanausahakul, R;Dietzschold, B
通讯作者：
Dietzschold, B
影响因子：
4.2
作者：
Kuzmin, I. V.;Hughes, G. J.;Rupprecht, C. E.
通讯作者：
Rupprecht, C. E.