Characterization of a phytoplasma associated with witches' broom disease of potatoes in Korea

Characterization of a phytoplasma associated with witches' broom disease of potatoes in Korea
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韩国马铃薯巫帚病相关植原体的特征

DOI:
10.1007/s10327-002-0012-0
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发表时间:
2003
影响因子:
1.2
通讯作者:
S. Namba
S. Namba
中科院分区:
农林科学4区
文献类型:
--
作者:
H. Jung;Y. Hahm;Joon;T. Hibi;S. Namba

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摘要1998年,在韩国济州岛的一个仓库中,在马铃薯(Solanum tuberosum L.)中观察到由植原体引起的巫秆病的症状,包括发育迟缓和发黄。利用通用植物原体引物对PCR扩增的16S核糖体DNA和16S- 23s间隔区DNA产物进行序列分析,鉴定出与马铃薯丛枝病(PWB)相关的植物原体属于16S- VIII族。与榆树AH植原体亲缘关系最密切,相似度达99.7%。AF268895),属于三叶草增殖(CP)亚群。这是东亚大陆首次发现CP亚群植物致病性植物原体,并包含了大部分马铃薯植物原体16S rDNA的核苷酸序列。
Abstract Symptoms of witches' broom disease caused by phytoplasma, including general stunting and yellowing, were observed in potatoes (Solanum tuberosum L.) in a storehouse on Jeju Island, Korea in 1998. Based on sequence analysis of DNA products from polymerase chain reaction (PCR)-amplified 16S ribosomal DNA and 16S–23S spacer region using universal phytoplasma primers, the phytoplasma associated with potato witches' broom disease (PWB) was identified as a member of 16S-group VIII. It was most closely related to elm AH phytoplasma (99.7% similarity, accession no. AF268895), which is in the clover proliferation (CP) subgroup. This report is the first from the East Asian continent of a plant pathogenic phytoplasma belonging to the CP subgroup and includes the nucleotide sequence of most of the potato phytoplasma 16S rDNA.
DOI: --
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DOI: 10.1099/ijs.0.02393-0
发表时间: 2003-07-01
影响因子: 2.8
作者:
Jung, HY;Sawayanagi, T;Namba, S
通讯作者: Namba, S