Tyrosine and carboxyl protonation changes in the bacteriorhodopsin photocycle. 1. M412 and L550 intermediates.
Tyrosine and carboxyl protonation changes in the bacteriorhodopsin photocycle. 1. M412 and L550 intermediates.
复制标题
细菌视紫红质光循环中酪氨酸和羧基质子化的变化。
作者：
P. Roepe;P. Ahl;S. K. Gupta;J. Herzfeld;K. Rothschild
已经通过使用傅立叶变换红外（FTIR）和紫外线差异光谱法研究了酪氨酸在细菌紫红素（BR）光循环中的作用。酪氨酸对在几个温度下记录的BR570 ---- M412 FTIR差异谱图通过同位素标记细菌淡季中的酪氨酸残基来鉴定。这些峰的频率和氘/氢交换敏感性和模型化合物在几种环境中的光谱中的峰表明，在M412形成期间，至少两个不同的酪氨酸基团参与了BR光循环。一组在BR570 ---- K630过渡期间经历了酪氨酸的酪氨酸转换。第二个酪氨酸群在L550至M412之间。紫色膜悬浮液和再水化膜的220--350-nm区域中的低温紫外差异光谱支持这些结论。紫外线光谱还表明一个或多个色氨酸组的扰动。如1770 - 1720-CM-1区域的红外吸收变化所示，几个羧基似乎在BR570和M412之间发生了一系列质子化变化。这些结果与涉及酪氨酸和羧基的细菌视紫红质中的质子线的存在一致。
The role of tyrosines in the bacteriorhodopsin (bR) photocycle has been investigated by using Fourier transform infrared (FTIR) and UV difference spectroscopies. Tyrosine contributions to the BR570----M412 FTIR difference spectra recorded at several temperatures and pH's were identified by isotopically labelling tyrosine residues in bacteriorhodopsin. The frequencies and deuterium/hydrogen exchange sensitivities of these peaks and of peaks in spectra of model compounds in several environments suggest that at least two different tyrosine groups participate in the bR photocycle during the formation of M412. One group undergoes a tyrosinate----tyrosine conversion during the BR570----K630 transition. A second tyrosine group deprotonates between L550 and M412. Low-temperature UV difference spectra in the 220--350-nm region of both purple membrane suspensions and rehydrated films support these conclusions. The UV spectra also indicate perturbation(s) of one or more tryptophan group(s). Several carboxyl groups appear to undergo a series of protonation changes between BR570 and M412, as indicated by infrared absorption changes in the 1770--1720-cm-1 region. These results are consistent with the existence of a proton wire in bacteriorhodopsin that involves both tyrosine and carboxyl groups.
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Meta II 衰变过程中视紫红质重折叠的证据。
影响因子：
3.4
作者：
Rothschild,KJ;Gillespie,J;DeGrip,WJ
通讯作者：
DeGrip,WJ
DOI：
10.1073/pnas.79.2.403
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BRAIMAN, M;MATHIES, R
通讯作者：
MATHIES, R
影响因子：
16.6
作者：
W. Stoeckenius;R. Bogomolni
通讯作者：
W. Stoeckenius;R. Bogomolni
DOI：
10.1073/pnas.83.2.347
发表时间：
1986
影响因子：
11.1
作者：
K. Rothschild;P. Roepe;P. Ahl;T. Earnest;R. Bogomolni;S. K. Gupta;C. M. Mulliken;J. Herzfeld
通讯作者：
K. Rothschild;P. Roepe;P. Ahl;T. Earnest;R. Bogomolni;S. K. Gupta;C. M. Mulliken;J. Herzfeld
细菌视紫红质及其用氘代酪氨酸再生的光产物的傅里叶变换红外差异光谱。
影响因子：
2.9
作者：
Dollinger,G;Eisenstein,L;Lin,SL;Nakanishi,K;Termini,J
通讯作者：
Termini,J