Alleviation of myocardial stunning by leukocyte and platelet depletion.
Alleviation of myocardial stunning by leukocyte and platelet depletion.
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通过白细胞和血小板消耗减轻心肌顿抑。
DOI：
10.1161/01.cir.80.6.1828
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发表时间：
1989
期刊：
影响因子：
37.8
通讯作者：
Mullane,KM
中科院分区：
文献类型：
--
作者：
Westlin,W;Mullane,KM
中性粒细胞聚集在心肌缺血和再灌注。活化的中性粒细胞释放介质，如氧代谢产物，可损害肌细胞的完整性并引起心功能障碍。虽然已经确定白细胞减少可减少梗死面积，但白细胞和自由基来源在缺血后收缩功能障碍中的作用仍未得到解决。在缺血前30分钟，在麻醉开胸犬中建立颈动脉左前降支冠状动脉体外回路，不使用Leukopak过滤器（n = 8）或使用Leukopak过滤器（n = 6）以从进入左前降支动脉的血液中去除白细胞和血小板。用声显微测量法监测肺动脉瓣下节段功能，通过停流15分钟，然后再灌注3小时来产生缺血。在再灌注15分钟时，白细胞消耗90 +/- 3.2%和血小板消耗100%改善了节段性功能（对照组与白细胞消耗组相比，分别从30.5 +/- 7%改善至74.1 +/- 12.7%）。然而，在白细胞减少组中，再灌注3小时时收缩力逐渐下降至32.5 +/- 13.8%，这与白细胞的恢复有关，体外血液中血小板的恢复程度较低，分别为全身值的70.2 +/- 21.9%和15.5 +/- 4.3%。仅在再灌注时从灌注的冠状血管床的血液中去除白细胞和血小板，在15分钟时将收缩功能改善至67.7 +/- 6.9%，在3小时时改善至54.7 +/- 12.1%（n = 6）。扫描电子显微镜检查显示，再灌注3小时后，对照动物的心外膜冠状动脉中有粘附的白细胞。（250字处删节）
Neutrophils accumulate in myocardium rendered ischemic and reperfused. Activated neutrophils release mediators such as metabolites of oxygen that can compromise myocellular integrity and provoke cardiac dysfunction. Although it is established that leukopenia reduces infarct size, the role of leukocytes and the source of free radicals in postischemic contractile dysfunction is unresolved. A carotid left anterior descending coronary-artery extracorporeal circuit without (n = 8) or with a Leukopak filter (n = 6) to deplete the leukocytes and platelets from blood entering the left anterior descending artery was established in the anesthetized, open-chest dog 30 minutes before ischemia. Subendocardial segmental function was monitored by sonomicrometry, and ischemia was produced by stopping flow for 15 minutes followed by 3 hours of reperfusion. Depleting leukocytes by 90 +/- 3.2% and platelets by 100% improved segmental function (from 30.5 +/- 7% to 74.1 +/- 12.7% for control versus leukocyte-depleted dogs, respectively) at 15 minutes of reperfusion. In the leukopenic group, however, there was a progressive decline in contractility to 32.5 +/- 13.8% by 3 hours of reperfusion that was associated with a return of leukocytes and, to a lesser extent, a return of platelets in the extracorporeal blood to 70.2 +/- 21.9% and 15.5 +/- 4.3% of systemic values, respectively. Removal of leukocytes and platelets from blood perfusing the coronary vascular bed only at reperfusion improved contractile function to 67.7 +/- 6.9% at 15 minutes and 54.7 +/- 12.1% at 3 hours (n = 6). Scanning electron microscopy revealed adherent leukocytes in the epicardial coronary arteries of control animals after 3 hours of reperfusion.(ABSTRACT TRUNCATED AT 250 WORDS)
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影响因子：
--
作者：
J. Baker;G. Bullock;K. Butler;I. Williamson;A. White
通讯作者：
A. White
影响因子：
15.9
作者：
BOLLI, R;PATEL, BS;MCCAY, PB
通讯作者：
MCCAY, PB
影响因子：
15.9
作者：
HEYNDRICKX, GR;MILLARD, RW;VATNER, SF
通讯作者：
VATNER, SF
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影响因子：
10.8
作者：
O'Neill,PG;Charlat,ML;Kim,HS;Pocius,J;Michael,LH;Hartley,CJ;Zhu,WX;Roberts,R;Bolli,R
通讯作者：
Bolli,R
DOI：
10.1152/ajpheart.1989.256.2.h341
发表时间：
1989-02
期刊：
The American journal of physiology
影响因子：
--
作者：
P. O’Neill;M. L. Charlat;L. Michael;R. Roberts;R. Bolli
通讯作者：
P. O’Neill;M. L. Charlat;L. Michael;R. Roberts;R. Bolli