CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis.
CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis.
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DOI：
10.1371/journal.pone.0052144
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nixon DF
中科院分区：
文献类型：
--
作者：
Batista MD;Tincati C;Milush JM;Ho EL;Ndhlovu LC;York VA;Kallas EG;Kalil J;Keating SM;Norris PJ;Chang D;Unemori P;Leslie KS;Maurer T;Liao W;Nixon DF
导致银屑病的免疫病理机制尚未解决。CD 57是CD 8 + T细胞上的复制无能和免疫衰老的标志物，并且表达CD 57的CD 8 + T细胞的比例在许多炎性病症中增加。我们检测了银屑病患者皮肤中T细胞上CD 57的表达，比较了皮损和未受影响的皮肤。我们还通过评估从银屑病患者的病变和未受影响的皮肤活检分离的细胞分选的纯化的CD 4+和CD 8 + T细胞中分泌的几种炎性细胞因子（IL-17 A、IFN-γ、IL-2、IL-33、TNF-α、IL-21、IL-22和IL-27）来评估T细胞的功能。我们观察到，与皮损皮肤相比，银屑病患者未受影响的皮肤中CD 57 + CD 4+和CD 57 + CD 8 + T细胞的频率显著更高。与未受影响的皮肤相比，来自银屑病皮损的分选的CD 4 + T细胞产生更高水平的IL-17 A、IL-22和IFN-γ，而与未受影响的皮肤相比，来自皮损的分选的CD 8 + T细胞产生更高水平的IL-17、IL-22、IFN-γ、TNF-α和IL-2。这些发现表明，银屑病患者未受影响的皮肤中的T细胞表现出与复制能力丧失相容的表型。由于它们具有较低的复制能力，CD 57 + T细胞由于高细胞更新而在病变组织中不太常见。
The immunopathogenic mechanisms leading to psoriasis remain unresolved. CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions. We examined the expression of CD57 on T cells in the skin of patients affected with psoriasis, comparing lesional and unaffected skin. We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients. We observed that the frequency of CD57+CD4+ and CD57+CD8+ T cells was significantly higher in unaffected skin of psoriasis patients compared to lesional skin. Sorted CD4+ T cells from psoriatic lesional skin produced higher levels of IL-17A, IL-22, and IFN-gamma compared to unaffected skin, while sorted CD8+ T cells from lesional skin produced higher levels of IL-17, IL-22, IFN-gamma, TNF-alpha, and IL-2 compared to unaffected skin. These findings suggest that T cells in unaffected skin from psoriasis patients exhibit a phenotype compatible with replicative inability. As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover.
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DOI：
10.1016/j.jaci.2010.12.1124
发表时间：
2011-04
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Suárez-Fariñas M;Tintle SJ;Shemer A;Chiricozzi A;Nograles K;Cardinale I;Duan S;Bowcock AM;Krueger JG;Guttman-Yassky E
通讯作者：
Guttman-Yassky E
影响因子：
5.5
作者：
Ortega, Consuelo;Fernandez-A, Silvia;Santamaria, Manuel
通讯作者：
Santamaria, Manuel
影响因子：
3
作者：
Cameron, AL;Kirby, B;Griffiths, CEM
通讯作者：
Griffiths, CEM
影响因子：
14.2
作者：
Nograles, Kristine E.;Zaba, Lisa C.;Shemer, Avner;Fuentes-Duculan, Judilyn;Cardinale, Irma;Kikuchi, Toyoko;Ramon, Michal;Bergman, Reuven;Krueger, James G.;Guttman-Yassky, Emma
通讯作者：
Guttman-Yassky, Emma
影响因子：
20.3
作者：
Brenchley, JM;Karandikar, NJ;Koup, RA
通讯作者：
Koup, RA