Pollen factors controlling self-incompatibility strength in Japanese pear
Pollen factors controlling self-incompatibility strength in Japanese pear
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控制日本梨自交不亲和强度的花粉因子
DOI：
10.1007/s00497-012-0202-7
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发表时间：
2012
影响因子：
--
通讯作者：
K. Nada
中科院分区：
文献类型：
--
作者：
S. Hiratsuka;M. Fujimura;T. Hayashida;Yutaka Nishikawa;K. Nada
日本梨具有遗传控制的自交不亲和系统，但半活体花粉管生长和自花授粉后坐果率在不同品种之间存在较大差异。花柱中花粉管达到基数的百分比为0-36.5%，这一值由花柱的培养和座果率决定，为0.6-15.2%。根据这些数据，我们为每个品种的自交不亲和性弱值赋值。在这里，我们证明了花粉因素控制着自交不亲和的程度。将13个品种的花粉管生长按完全亲和的‘厚生’(S1S7)风格进行比较，品种间差异较大，与自交不亲和弱度呈极显著正相关(r=-0.707)。因此，梨的自交不亲和程度是由与该基因座无关的花粉因子(S)决定的。花粉供体对‘厚生’坐果率和果实生长无明显影响，但种子数与自交不亲和性呈显著正相关(r=0.0.972)。而在有NOS-RNase产生的花柱(S4smS4sm)中，这种关系消失(r=0.341)，花粉管生长除1个品种外，其余均提高了12-36%。这些结果表明，核糖核酸酶在亲和花粉上以一种细胞毒素的方式发挥作用，其抑制程度与品种有关，抑制程度由与该基因座无关的花粉因子(S)决定。花粉因子也在不亲和的花柱中作用于ONS-RNase，导致不同程度的自交不亲和。
Japanese pear has a genetically controlled self-incompatibility system, but both the pollen-tube growth in a semi in vivo assay and fruit set after self-pollination differ considerably among cultivars. The percentage of styles in which pollen tubes have reached the base ranges from 0 to 36 %, a value determined by culture of styles in vitro, and fruit set ranges from 0.6 to 15.2 %. Based on these data, we have assigned a value for the self-incompatibility weakness to each cultivar. Here, we showed that pollen factors control the degree of self-incompatibility. When the pollen-tube growth of 13 cultivars was compared in a completely compatible ‘Hougetsu’ (S1S7) style, it differed a fair amount among cultivars and showed a significantly positive relation to self-incompatibility weakness (r= 0.707). The degree of self-incompatibility of pear is, therefore, determined by pollen factor(s) unrelated to theS-locus. Although the fruit set and fruit growth of ‘Hougetsu’ were not affected by the pollen donor, a positive relationship was also observed between seed number and self-incompatibility weakness (r= 0.972). However, in a style with noS-RNase production (genotype:S4smS4sm), the relationship disappeared (r= 0.341) and pollen-tube growth was promoted by 12–36 % except in one cultivar. These results suggest thatS-RNase functions as a cytotoxin on compatible pollen in a cultivar-dependent manner, and that the degree of inhibition is determined by pollen factor(s) unrelated to theS-locus. The pollen factor also functions onS-RNase in incompatible styles, resulting in a different degree of self-incompatibility.
影响因子：
5.4
作者：
H. Yamane;K. Ushijima;H. Sassa;R. Tao
通讯作者：
H. Yamane;K. Ushijima;H. Sassa;R. Tao