The alternative sigma factor RpoD4 pulses at division and links the clock and the cell cycle in Synechococcus elongatus
The alternative sigma factor RpoD4 pulses at division and links the clock and the cell cycle in Synechococcus elongatus
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细长聚球藻中替代 sigma 因子 RpoD4 在分裂时发出脉冲并将时钟和细胞周期联系起来
DOI：
10.1101/2023.06.28.546855
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Ye C
中科院分区：
文献类型：
--
作者：
Ye C
The cyanobacterial circadian clock is an extensive regulator of gene expression, generating a 24-hour rhythm in the majority of genes in Synechococcus elongatus PCC 7942. This raises the question of how the cyanobacterial clock regulates, and receives input from, the diverse cellular processes it controls. Alternative sigma factors, which bind to the core RNA polymerase enzyme and direct the synthesis of specific sets of transcripts, are one mechanism for the clock to set the time of its outputs. In this work, we use single-cell time-lapse microscopy to reveal the transcriptional dynamics of RpoD4, an alternative sigma factor that was previously reported as either arrhythmic or circadian. We find instead that RpoD4 pulses at cell division, dynamics missed by previous bulk averaging. The circadian clock modulates the amplitude of RpoD4 expression pulses, as well as the timing of pulses through its control of division timing. In turn, a rpoD4 mutation causes a reduction in clock period. Further, a rpoD4 mutant results in smaller cell size, and increasing expression of RpoD4 results in larger cell sizes in a dose-response manner, allowing tunable control of cell size. Thus, our single-cell analysis has revealed pulsing gene expression dynamics in cyanobacteria, linking the clock, RpoD4 and the cell cycle.
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DOI：
10.1073/pnas.1812871115
发表时间：
2018-12
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Yiling Yang;Vinson Lam;M. Adomako;Ryan Simkovsky;A. Jakob;N. Rockwell;Susan E. Cohen;Susan E. Cohen;A. Taton;Jingtong Wang;J. Lagarias;A. Wilde;David Nobles;J. Brand;S. Golden
通讯作者：
Yiling Yang;Vinson Lam;M. Adomako;Ryan Simkovsky;A. Jakob;N. Rockwell;Susan E. Cohen;Susan E. Cohen;A. Taton;Jingtong Wang;J. Lagarias;A. Wilde;David Nobles;J. Brand;S. Golden
检测蓝藻中荧光素酶报告基因的节律性生物发光。
DOI：
10.1007/978-1-59745-257-1_8
发表时间：
2007
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Mackey,ShannonR;Ditty,JaynaL;Clerico,EugeniaM;Golden,SusanS
通讯作者：
Golden,SusanS
高光诱导 2 组 sigma 因子 RpoD3 及其在细长聚球藻 PCC 7942 中的潜在机制
DOI：
--
发表时间：
2007
期刊：
J. Biol. Chem 281
影响因子：
--
作者：
A. Seki;M. Hanaoka;Y. Akimoto;S. Masuda;H. Iwasaki;K. Tanaka
通讯作者：
K. Tanaka
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
L. C. L. Palmer-L.-C.-L.-Palmer-2245994539;J. M. Cotton;Henley;Y Zheng;J. Mellem;P. J. Brockie;D. M. Madsen;Y Stern-Bach;S. Russo;M. Neuman;Christian Rosenmund;N Pilpel;N. Landeck;M. Klugmann;P. Seeburg;M. K. Schwarz;K M Partin;M. W. Fleck;M. L. Mayer;A Robert;N. Armstrong;J. Gouaux;J. R. Howe;M C Weston;C. Gertler;R. Hinz-Herkommer;I. Preugschat-Gumprecht;R. Zilberstein;Y. S. B. Sfb;Nationales Genomforschungsnetz;Max Planck Society;P. H. S. The;Qiong Yang;B. Pando;Guogang Dong;S. Golden;A. van Oudenaarden
通讯作者：
L. C. L. Palmer-L.-C.-L.-Palmer-2245994539;J. M. Cotton;Henley;Y Zheng;J. Mellem;P. J. Brockie;D. M. Madsen;Y Stern-Bach;S. Russo;M. Neuman;Christian Rosenmund;N Pilpel;N. Landeck;M. Klugmann;P. Seeburg;M. K. Schwarz;K M Partin;M. W. Fleck;M. L. Mayer;A Robert;N. Armstrong;J. Gouaux;J. R. Howe;M C Weston;C. Gertler;R. Hinz-Herkommer;I. Preugschat-Gumprecht;R. Zilberstein;Y. S. B. Sfb;Nationales Genomforschungsnetz;Max Planck Society;P. H. S. The;Qiong Yang;B. Pando;Guogang Dong;S. Golden;A. van Oudenaarden
影响因子：
16.6
作者：
Johnson CH;Egli M
通讯作者：
Egli M