Smooth muscle cells freshly isolated from rat thoracic aortas are much stiffer than cultured bovine cells : Possible effect of phenotype
Smooth muscle cells freshly isolated from rat thoracic aortas are much stiffer than cultured bovine cells : Possible effect of phenotype
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从大鼠胸主动脉中新鲜分离的平滑肌细胞比培养的牛细胞僵硬得多：表型的可能影响
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
Masaaki Sato
中科院分区：
文献类型：
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作者：
Takeo Matsumoto;J. Sato;Mitsunobu Yamamoto;Masaaki Sato
在37°C生理盐溶液中获得了经酶切分离的大鼠主动脉平滑肌细胞（RASMs）的拉伸性能，并与培养的牛主动脉平滑肌细胞（BASMs, P6-7）进行了比较。应变速率为0.2 ~ 4%/s时，rasm和basm的总弹性模量分别为9.3±2.8 kPa（平均±SEM， n=8）和1.5±0.2 kPa （n=6）。由于细胞制备方法的不同，我们认为rasm和basm分别表现为收缩表型和合成表型。肌丝中的收缩细胞比合成细胞丰富得多。肌丝束可能比其他细胞成分更硬，并延伸到整个细胞质。因此，收缩细胞可能比合成细胞更硬。这些细胞力学性能的差异可能是由于两种细胞标本表型的差异。
Tensile properties of rat aortic smooth muscle cells (RASMs) freshly isolated by enzymatic digestion were obtained under a physiological salt solution at 37°C and compared with those of cultured bovine aortic smooth muscle cells (BASMs, P6-7). Overall elastic modulus of RASMs and BASMs were 9.3±2.8 kPa (mean±SEM, n=8) and 1.5±0.2 kPa (n=6), respectively under the strain rate of 0.2-4%/s. Due to the difference in the cell preparation method, RASMs and BASMs are considered to show contractile and synthetic phenotype, respectively. Contractile cells are much more abundant in myofilaments than synthetic cells. Myofilament bundles may be stiffer than other cellular components and extends throughout the cytoplasm. Contractile cells may thus be stiffer than synthetic cells. The difference in the mechanical properties of these cells may be due to the difference in the phenotype of the two cell specimens.
DOCA加盐高血压大鼠动脉壁的时程变化。
DOI：
10.1161/01.hyp.4.1.27
发表时间：
1982
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
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作者：
Cox,RH
通讯作者：
Cox,RH