Diversity of blaCTX-M-1-carrying plasmids recovered from Escherichia coli isolated from Canadian domestic animals.
Diversity of blaCTX-M-1-carrying plasmids recovered from Escherichia coli isolated from Canadian domestic animals.
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DOI：
10.1371/journal.pone.0264439
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Boerlin P
中科院分区：
文献类型：
--
作者：
Cormier AC;Chalmers G;Zamudio R;Mulvey MR;Mather AE;Boerlin P
已发现携带blaCTX-M-1的保守IncI 1和IncHI 1质粒分别在欧洲大陆的鸡和马中循环。在加拿大，blaCTX-M-1是在鸡和马的大肠杆菌中发现的最常见的blaCTX-M变体，并且可以在猪，牛和狗中以较低的频率回收。全基因组测序已经确定了携带这种变体的分离株的大量遗传多样性，从而阻止了对携带这种基因的质粒的进一步调查。因此，本研究的目的是描述在大肠杆菌中循环的blaCTX-M-1质粒的遗传谱。大肠杆菌，并将其与欧洲动物中回收的大肠杆菌进行比较。51株blaCTX-M-1阳性E.选择来自鸡（n = 14）、马（赛马n = 11;社区马n = 3）、猪（n = 7）、火鸡（n = 6）、狗（n = 5）、肉牛（n = 3）和奶牛（n = 2）的大肠杆菌分离物用于质粒表征。通过Illumina和Oxford Nanopore技术获得序列。使用Unicycler杂交组装或Flye组装基因组，使用Pilon进行抛光。blaCTX-M-1在45株分离株中位于质粒上，在6株分离株中位于染色体上。在鸡（n = 12）、火鸡（n = 4）、猪（n = 6）、狗（n = 2）和肉牛（n = 2）分离株中鉴定出保守的IncI 1/ST 3质粒。当与公开数据比较时，这些质粒与欧洲家禽和猪分离株中鉴定的质粒高度相似。这些结果表明，与欧洲发现的类似的流行性IncI 1/ST 3质粒有助于blaCTX-M-1在加拿大的传播。还从几乎所有赛马分离株（n = 10）中回收了保守的IncHI 1/FIA（HI 1）/ST 2质粒。虽然IncHI 1/ST 2质粒已在欧洲马分离株中报告，但IncHI 1/ST 9质粒似乎更普遍。进一步的研究是必要的，以了解这些质粒的成功在各自的人群中的因素。
Conserved IncI1 and IncHI1 plasmids carrying blaCTX-M-1 have been found circulating in chickens and horses from continental Europe, respectively. In Canada, blaCTX-M-1 is overwhelmingly the most common blaCTX-M variant found in Escherichia coli from chicken and horses and can be recovered at lower frequencies in swine, cattle, and dogs. Whole-genome sequencing has identified a large genetic diversity of isolates carrying this variant, warranting further investigations into the plasmids carrying this gene. Therefore, the objective of this study was to describe the genetic profiles of blaCTX-M-1 plasmids circulating in E. coli from Canadian domestic animals and compare them to those recovered in animals in Europe. Fifty-one blaCTX-M-1 positive E. coli isolates from chicken (n = 14), horses (racetrack horses n = 11; community horses n = 3), swine (n = 7), turkey (n = 6), dogs (n = 5), beef cattle (n = 3), and dairy cattle (n = 2) were selected for plasmid characterization. Sequences were obtained through both Illumina and Oxford Nanopore technologies. Genomes were assembled using either Unicycler hybrid assembly or Flye with polishing performed using Pilon. blaCTX-M-1 was found residing on a plasmid in 45 isolates and chromosomally located in six isolates. A conserved IncI1/ST3 plasmid was identified among chicken (n = 12), turkey (n = 4), swine (n = 6), dog (n = 2), and beef cattle (n = 2) isolates. When compared against publicly available data, these plasmids showed a high degree of similarity to those identified in isolates from poultry and swine in Europe. These results suggest that an epidemic IncI1/ST3 plasmid similar to the one found in Europe is contributing to the spread of blaCTX-M-1 in Canada. A conserved IncHI1/FIA(HI1)/ST2 plasmid was also recovered from nearly all racetrack horse isolates (n = 10). Although IncHI1/ST2 plasmids have been reported among European horse isolates, IncHI1/ST9 plasmids appear to be more widespread. Further studies are necessary to understand the factors contributing to these plasmids’ success in their respective populations.
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影响因子：
3.3
作者：
Cormier, Ashley;Zhang, Pauline L. C.;Boerlin, Patrick
通讯作者：
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影响因子：
5.2
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Archambault, M
影响因子：
5.2
作者：
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Carattoli, Alessandra