Synthesis and Evaluation of Europium Complexes that Switch on Luminescence in Lysosomes of Living Cells.
Synthesis and Evaluation of Europium Complexes that Switch on Luminescence in Lysosomes of Living Cells.
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DOI：
10.1002/chem.202003992
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发表时间：
2021-01-07
期刊：
影响因子：
--
通讯作者：
Parker D
中科院分区：
文献类型：
--
作者：
Starck M;Fradgley JD;Pal R;Zwier JM;Lamarque L;Parker D
研究了一组四种带有扩展芳基-炔基吡啶发色团的发光EuIII络合物，在其吸收和发射光谱响应中显示出非常不同的pH依赖性行为。对于在含蛋白质溶液中pKa值为6.45和6.20的两种复合物，质子化后发射寿命非常显著地增加。通过在信号采集期间改变选通时间，可以优化"接通"强度比，并且在pH 8和4之间测量到250至1330之间的增强因子。 表现最好的探针显示对内源性阳离子、还原剂和血清白蛋白的浓度没有显著的发射依赖性。在活细胞成像研究中对其进行了检查，以监测时间依赖性溶酶体酸化，在NIH-3T3细胞中，由于酸化而观察到的图像亮度增加了50倍。 通过使用四种具有延伸的芳基-烷基吡啶天线的铕络合物，显示出非常不同的pH依赖性行为，可以用"开启"光学传感器观察溶酶体老化。铕发光在低pH值下发生"开启"，使共聚焦显微镜能够在活细胞成像研究中显示老化溶酶体的图像亮度增加。
A set of four luminescent EuIII complexes bearing an extended aryl‐alkynylpyridine chromophore has been studied, showing very different pH‐dependent behaviour in their absorption and emission spectral response. For two complexes with pK a values of 6.45 and 6.20 in protein‐containing solution, the emission lifetime increases very significantly following protonation. By varying the gate time during signal acquisition, the ‘switch‐on’ intensity ratio could be optimised, and enhancement factors of between 250 to 1330 were measured between pH 8 and 4. The best‐behaved probe showed no significant emission dependence on the concentration of endogenous cations, reductants, and serum albumin. It was examined in live‐cell imaging studies to monitor time‐dependent lysosomal acidification, for which the increase in observed image brightness due to acidification was a factor of 50 in NIH‐3T3 cells. Watching lysosomes age with a ‘switch‐on’ optical sensor is shown to be possible by using four europium complexes with extended aryl‐alkylnyl pyridine antennae that show very different pH‐dependent behaviour. A ‘switch‐on’ of europium luminescence occurs at low pH, allowing confocal microscopy to reveal increases in image brightness of ageing lysosomes in live‐cell imaging studies.
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影响因子：
1.8
作者：
Kovacs, Z;Sherry, AD
通讯作者：
Sherry, AD
细胞显微镜和基于荧光的细胞内细胞器中的腔pH测量。
影响因子：
5.5
作者：
Ma L;Ouyang Q;Werthmann GC;Thompson HM;Morrow EM
通讯作者：
Morrow EM
影响因子：
13.3
作者：
Ponsford AH;Ryan TA;Raimondi A;Cocucci E;Wycislo SA;Fröhlich F;Swan LE;Stagi M
通讯作者：
Stagi M
影响因子：
4
作者：
Butler, Stephen J.;Delbianco, Martina;Zwier, Jurriaan M.
通讯作者：
Zwier, Jurriaan M.
影响因子：
5.2
作者：
Levoye A;Zwier JM;Jaracz-Ros A;Klipfel L;Cottet M;Maurel D;Bdioui S;Balabanian K;Prézeau L;Trinquet E;Durroux T;Bachelerie F
通讯作者：
Bachelerie F