Comparison of the Genital Microbiomes of Pregnant Aboriginal and Non-aboriginal Women.
Comparison of the Genital Microbiomes of Pregnant Aboriginal and Non-aboriginal Women.
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DOI：
10.3389/fcimb.2020.523764
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发表时间：
2020
影响因子：
5.7
通讯作者：
Mendz GL
中科院分区：
文献类型：
--
作者：
Dinsdale NK;Castaño-Rodríguez N;Quinlivan JA;Mendz GL
女性的生殖器微生物群因种族背景而异。早产和早发性新生儿败血症是与妊娠期生殖器感染相关的两种结果。澳大利亚土著母亲的早产率很高，婴儿的早发性败血症率也很高。到目前为止，还没有对这组妇女进行生殖器微生物群相关感染的研究。在西澳大利亚的黑德兰健康校园进行了一项前瞻性队列研究，以表征来自皮尔巴拉地区的一组妇女的阴道和胎盘微生物组。本研究包括孕妇土著（n = 23）和非土著（n = 27）分娩或计划进行子宫下段剖宫产的妇女。在阴道分娩或计划剖腹产之前，在无菌条件下使用无菌拭子获取阴道样本；并在分娩过程中，在相同条件下采集胎盘样本。通过16S rRNA扩增子测序（V4区，515F-806R）对样品中的分类群进行鉴定。分类单元（Operational Taxonomic Unit， OTU）计数确定了分类单元的身份和丰度。统计分析结合临床元数据和测序结果来确定分类群与种族背景的关系。这项工作的发现有助于加强目前对与土著和非土著妇女健康和疾病相关的微生物群的理解。原住民和非原住民妇女在怀孕期间的阴道和胎盘微生物组存在差异，各种族群体中特定分类群的丰度也存在显著差异。特定类群的相对丰度在有感染临床体征的受试者和健康妊娠的受试者之间有显著差异。这项工作将有助于了解不同种族人群中感染驱动的早产率差异的原因。
The genital microbiomes of women varies with racial background. Preterm birth and early-onset neonatal sepsis are two outcomes associated with genital infections during pregnancy. The rate of preterm birth in Aboriginal Australian mothers is high, as is the rate of early-onset sepsis in their infants. To date, no studies have been conducted to investigate genital microbiome taxa associated infection in this group of women. A prospective cohort study to characterize the vaginal and placental microbiomes of a group of these women from the Pilbara region was conducted at the Hedland Health Campus in Western Australia. Included in the study were gravidae Aboriginal (n = 23) and Non-aboriginal (n = 27) women in labor or for planned lower uterine segment Caesarean section. Employing sterile swabs, vaginal samples were obtained under sterile conditions immediately prior to vaginal delivery or planned Caesarean section; and placental samples were obtained under the same conditions during labor. Taxa present in the samples were identified by 16S rRNA amplicon sequencing (V4 region, 515F-806R). Taxon identity and abundance were established from Operational Taxonomic Unit (OTU) counts. Statistical analyses combining clinical metadata and sequencing results were employed to determine associations of taxa with racial background. The findings of this work served to enhance the current understanding of microbiota associated with health and disease in Aboriginal and Non-Aboriginal women. Differences were found between the vaginal and placental microbiomes of Aboriginal and Non-aboriginal women during pregnancy, as well as notable differences between the abundance of specific taxa in each racial group. The relative abundances of specific taxa were significantly different between participants with clinical signs of infection and those with healthy pregnancies. This work will contribute to understanding the causes of differences in rates of infection-driven preterm birth in various racial populations.
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影响因子：
3.7
作者：
Aagaard K;Riehle K;Ma J;Segata N;Mistretta TA;Coarfa C;Raza S;Rosenbaum S;Van den Veyver I;Milosavljevic A;Gevers D;Huttenhower C;Petrosino J;Versalovic J
通讯作者：
Versalovic J
影响因子：
17.1
作者：
Gajer P;Brotman RM;Bai G;Sakamoto J;Schütte UM;Zhong X;Koenig SS;Fu L;Ma ZS;Zhou X;Abdo Z;Forney LJ;Ravel J
通讯作者：
Ravel J
DOI：
10.1073/pnas.1502875112
发表时间：
2015-09-01
影响因子：
11.1
作者：
DiGiulio, Daniel B.;Callahan, Benjamin J.;Relman, David A.
通讯作者：
Relman, David A.
影响因子：
3.1
作者：
Franasiak, J. M.;Werner, M. D.;Scott, R. T.
通讯作者：
Scott, R. T.
影响因子：
7
作者：
Goltsman DSA;Sun CL;Proctor DM;DiGiulio DB;Robaczewska A;Thomas BC;Shaw GM;Stevenson DK;Holmes SP;Banfield JF;Relman DA
通讯作者：
Relman DA