Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats.
Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats.
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DOI：
10.1111/mec.15724
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发表时间：
2021-07
影响因子：
4.9
通讯作者：
Rossiter SJ
中科院分区：
文献类型：
--
作者：
Drinkwater R;Jucker T;Potter JHT;Swinfield T;Coomes DA;Slade EM;Gilbert MTP;Lewis OT;Bernard H;Struebig MJ;Clare EL;Rossiter SJ
将元条形码应用于环境和无脊椎动物来源的DNA（eDNA和iDNA）是一种新的且越来越多地应用于监测各种栖息地生物多样性的方法。这种方法特别适用于生物多样性潮湿热带地区的采样，在那里，农业土地利用的快速变化意味着越来越需要了解剩余马赛克和退化景观的保护价值。在这里，我们使用来自吸血水蛭（Haemadipsa picta）的iDNA来评估马来西亚婆罗洲沙巴森林退化梯度中哺乳动物多样性的差异。我们筛选了557个个体水蛭的哺乳动物DNA的16S rRNA基因的靶向片段，并检测到14个哺乳动物属。我们记录了较低的哺乳动物多样性，在最严重的退化森林相比，高质量的两次采伐森林。虽然累积曲线的多样性估计在这些栖息地类型是可比的，多样性较高，在两次采伐森林，更多的类群的保护问题。此外，我们的分析揭示了记录在重度采伐森林和两次采伐森林的社区之间的差异。通过揭示人类改造的热带景观中哺乳动物多样性的差异，我们的研究证明了iDNA作为保护评估中的非侵入性生物监测方法的价值。
The application of metabarcoding to environmental and invertebrate‐derived DNA (eDNA and iDNA) is a new and increasingly applied method for monitoring biodiversity across a diverse range of habitats. This approach is particularly promising for sampling in the biodiverse humid tropics, where rapid land‐use change for agriculture means there is a growing need to understand the conservation value of the remaining mosaic and degraded landscapes. Here we use iDNA from blood‐feeding leeches (Haemadipsa picta) to assess differences in mammalian diversity across a gradient of forest degradation in Sabah, Malaysian Borneo. We screened 557 individual leeches for mammal DNA by targeting fragments of the 16S rRNA gene and detected 14 mammalian genera. We recorded lower mammal diversity in the most heavily degraded forest compared to higher quality twice logged forest. Although the accumulation curves of diversity estimates were comparable across these habitat types, diversity was higher in twice logged forest, with more taxa of conservation concern. In addition, our analysis revealed differences between the community recorded in the heavily logged forest and that of the twice logged forest. By revealing differences in mammal diversity across a human‐modified tropical landscape, our study demonstrates the value of iDNA as a noninvasive biomonitoring approach in conservation assessments.
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影响因子：
4.9
作者：
Deagle BE;Thomas AC;McInnes JC;Clarke LJ;Vesterinen EJ;Clare EL;Kartzinel TR;Eveson JP
通讯作者：
Eveson JP
影响因子：
7.7
作者：
Dorazio, Robert M.;Erickson, Richard A.
通讯作者：
Erickson, Richard A.
影响因子：
4.9
作者：
Calvignac-Spencer, Sebastien;Merkel, Kevin;Leendertz, Fabian H.
通讯作者：
Leendertz, Fabian H.
DOI：
10.1098/rspb.2010.1062
发表时间：
2011-01-07
影响因子：
4.7
作者：
Edwards, David P.;Larsen, Trond H.;Wilcove, David S.
通讯作者：
Wilcove, David S.
影响因子：
5.9
作者：
Asner, Gregory P.;Brodrick, Philip G.;Coomes, David A.
通讯作者：
Coomes, David A.