MicroRNA evolution provides new evidence for a close relationship of Diplura to Insecta

MicroRNA evolution provides new evidence for a close relationship of Diplura to Insecta
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MicroRNA的进化为Diplura与昆虫纲的密切关系提供了新的证据

DOI:
10.1111/syen.12401
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发表时间:
2020-04
影响因子:
4.8
通讯作者:
Luan Yun-Xia
Luan Yun-Xia
中科院分区:
农林科学1区
文献类型:
--
作者:
Liu Ai-Min;Chen Wan-Jun;Huang Cheng-Wang;Qian Chang-Yuan;Liang Yan;Li Sheng;Zhan Shuai;Luan Yun-Xia

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四个六足动物谱系(Protura、弹尾动物、双足动物和昆虫动物)之间的系统发育相互关系对于理解昆虫的起源和六足动物的早期多样化至关重要,但根据现有数据很难澄清它们。在这项研究中,我们从 36 个泛节肢动物类群中鉴定出了 91 个保守的 microRNA (miRNA) 家族,其中包括 7 个新测序的非昆虫六足动物。我们发现主要的进化枝分化伴随着新的 miRNA 家族的起源,并且大多数 miRNA 簇都具有高度的微同线性。重要的是,我们能够鉴定出两个六足动物特有的 miRNA 家族,以及四个 miRNA 家族和一个仅存在于双足纲和昆虫纲中的 miRNA 簇,表明双足纲和昆虫纲之间存在密切关系以及六足纲的单系性。结合基于 miRNA 家族存在/不存在矩阵的系统发育分析,我们的研究证明了 miRNA 在解决深层系统发育问题方面的有效性。
The phylogenetic interrelationships among four hexapod lineages (Protura, Collembola, Diplura and Insecta) are pivotal to understanding the origin of insects and the early diversification of Hexapoda, but they have been difficult to clarify based on the available data. In this study, we identified 91 conserved microRNA (miRNA) families from 36 panarthropod taxa, including seven newly sequenced non‐insect hexapods. We found major clade differentiation accompanied by the origin of novel miRNA families, and most miRNA clusters are conserved with a high degree of microsynteny. Importantly, we were able to identify two miRNA families unique to Hexapoda, and four miRNA families and a miRNA cluster that exist exclusively in Diplura and Insecta, suggesting a close relationship between Diplura and Insecta as well as the monophyly of Hexapoda. Combined with a phylogenetic analysis based on the presence/absence matrix of miRNA families, our study demonstrates the effectiveness of miRNA in resolving deep phylogenetic problems.
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