DIM5/KMT1 controls fungal insect pathogenicity and genome stability by methylation of histone H3K4, H3K9 and H3K36.
DIM5/KMT1 controls fungal insect pathogenicity and genome stability by methylation of histone H3K4, H3K9 and H3K36.
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DIM5/KMT1 通过组蛋白 H3K4、H3K9 和 H3K36 的甲基化控制真菌昆虫致病性和基因组稳定性
DOI：
10.1080/21505594.2021.1923232
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发表时间：
2021-12
期刊：
影响因子：
5.2
通讯作者：
Feng MG
中科院分区：
文献类型：
--
作者：
Ren K;Mou YN;Tong SM;Ying SH;Feng MG
组蛋白H3 Lys 9，Lys 4和Lys 36（H3K_ME1/ME2/ME3）的单，DI-和三甲基化需要在真核生物中介导基于DNA的细胞事件所需）分别归类于KMT1，KMT2和KMT3家族。据报道，核在Beauveria bassiana中具有保守的和非规范的作用，这是一种绝缘性促进性真菌，是一种主要的dim5中断的主要来源。 /ME2，H3K9ME1/ME2和H3K36ME2。通过角质层 - 细胞感染（血液注射）以及在体内和体外生命周期至关重要的一系列细胞事件中，在病毒中严重损害，体内和体外，包括菌丝的生长，阻塞的分生作用，在体内造成了障碍，在体内造成了障碍，在体内造成了抑制，危害了碳水化合物的增殖，危害，细胞周期，降低的生物合成和降解酶的分泌以及对1,201的各种应力的敏感性与这些表型变化相关的基因失调基因（上/下降比：712：489），92（上/下比：59：33）编码转录因子以及参与翻译后修饰的蛋白质或酶可以充当这些转录活性基因对全球基因调节至关重要的首选标记。 B. bassiana的昆虫性生活方式和基因组稳定性。
ABSTRACT Mono-, di- and tri-methylation of histone H3 Lys 9, Lys 4, and Lys 36 (H3K_me1/me2/me3) required for mediation of DNA-based cellular events in eukaryotes usually rely upon the activities of histone lysine methyltransferases (KMTs) classified to the KMT1, KMT2, and KMT3 families, respectively. Here, an H3K9-specific DIM5/KMT1 orthologue, which lacks a C-terminal post-SET domain and localizes mainly in nucleus, is reported to have both conserved and noncanonical roles in methylating the H3 core lysines in Beauveria bassiana, an insect-pathogenic fungus serving as a main source of wide-spectrum fungal insecticides. Disruption of dim5 led to abolishment of H3K9me3 and marked attenuation of H3K4me1/me2, H3K9me1/me2 and H3K36me2. Consequently, the Δdim5 mutant lost the whole insect pathogenicity through normal cuticle infection, and was compromised severely in virulence through cuticle-bypassing infection (hemocoel injection) and also in a series of cellular events critical for the fungal virulence and lifecycle in vivo and in vitro, including reduced hyphal growth, blocked conidiation, impeded proliferation in vivo, altered carbohydrate epitopes, disturbed cell cycle, reduced biosynthesis and secretion of cuticle-degrading enzymes, and increased sensitivities to various stresses. Among 1,201 dysregulated genes (up/down ratio: 712:489) associated with those phenotypic changes, 92 (up/down ratio: 59:33) encode transcription factors and proteins or enzymes involved in posttranslational modifications, implying that the DIM5-methylated H3 core lysines could act as preferential marks of those transcription-active genes crucial for global gene regulation. These findings uncover a novel scenario of DIM5 and its indispensability for insect-pathogenic lifestyle and genome stability of B. bassiana.
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DOI：
10.1073/pnas.1809841115
发表时间：
2018-09-11
影响因子：
11.1
作者：
Li, Xueqin;Harris, C. Jake;Du, Jiamu
通讯作者：
Du, Jiamu
影响因子：
16.8
作者：
Bilokapic S;Strauss M;Halic M
通讯作者：
Halic M
影响因子：
16.6
作者：
Chong, Shin Yen;Cutler, Sam;Kao, Cheng-Fu
通讯作者：
Kao, Cheng-Fu
影响因子：
64.5
作者：
Guenther, Matthew G.;Levine, Stuart S.;Young, Richard A.
通讯作者：
Young, Richard A.
DOI：
10.1016/j.bbagen.2015.05.013
发表时间：
2015-09-01
影响因子：
3
作者：
Eram, Mohammad S.;Kuznetsova, Ekaterina;Vedadi, Masoud
通讯作者：
Vedadi, Masoud