Functional and molecular expression of the proton-coupled oligopeptide transporters in spleen and macrophages from mouse and human.
Functional and molecular expression of the proton-coupled oligopeptide transporters in spleen and macrophages from mouse and human.
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DOI：
10.1021/mp300700p
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发表时间：
2013-04-01
影响因子：
4.9
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Sun D;Wang Y;Tan F;Fang D;Hu Y;Smith DE;Jiang H
The aim of this study was to determine the expression and function of proton-coupled oligopeptide transporters (POTs) in spleen and macrophages, and their contribution to innate immune response induced by bacterial peptidomimetics γ-iE-DAP and MDP, Quantitative real-time PCR (qRT-PCR) and Western blot results revealed the mRNA and protein expression of PepT2, PhT1 and PhT2, but not PepT1, in the spleen of mice and human. In comparison to lymphocytes of the spleen, macrophages had higher transcript levels of PepT2 and PhT2. The cellular uptake of Ala-Lys-AMCA in mouse splenic macrophages was pH dependent with maximum uptake at pH 6.0, and the kinetic parameters were Km = 75.5 ± 14.3µM and Vmax = 25.4 ± 2.1 pmol/min per mg protein. The uptake of Ala-Lys-AMCA by mouse splenic macrophages was not inhibited by histidine, but was significantly inhibited by Glycyl-Sarcosine (GlySar) and carnosine (P<0.01), and by bacterial peptidomimetics γ-iE-DAP and MDP, ligands of nucleotide-binding oligomerization domain (NOD)-containing proteins. Carnosine and GlySar, but not histidine, attenuated the inflammatory response induced by γ-iE-DAP and MDP in mouse splenic macrophages. Functional expression of POTs was also demonstrated in THP-1 cells and dipeptides reduced the immune response induced by γ-iE-DAP. In conclusion, our findings are novel by providing important information on the molecular and functional expression of POTs in spleen. Moreover, it appears that the PepT2-mediated uptake of γ-iE-DAP and MDP in macrophages further contributes to the innate immune response.
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质子偶联寡肽转运蛋白（POT）在小鼠前列腺以及良性前列腺增生（BPH）和前列腺癌（PCa）患者中的表达
影响因子：
2.8
作者：
Sun, Dongli;Tan, Fuqing;Jiang, Huidi
通讯作者：
Jiang, Huidi
影响因子：
29.4
作者：
Dalmasso G;Nguyen HT;Ingersoll SA;Ayyadurai S;Laroui H;Charania MA;Yan Y;Sitaraman SV;Merlin D
通讯作者：
Merlin D
DOI：
10.4049/jimmunol.0802197
发表时间：
2009-04-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Marina-García N;Franchi L;Kim YG;Hu Y;Smith DE;Boons GJ;Núñez G
通讯作者：
Núñez G
影响因子：
3.9
作者：
Jappar, Dilara;Wu, Shu-Pei;Smith, David E.
通讯作者：
Smith, David E.
影响因子：
4.9
作者：
Hu Y;Smith DE;Ma K;Jappar D;Thomas W;Hillgren KM
通讯作者：
Hillgren KM