Amyloid structure and assembly: insights from scanning transmission electron microscopy.
Amyloid structure and assembly: insights from scanning transmission electron microscopy.
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DOI：
10.1016/j.jsb.2010.09.018
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发表时间：
2011-01
影响因子：
3
通讯作者：
Mueller, Shirley A.
中科院分区：
文献类型：
--
作者：
Goldsbury, Claire;Baxa, Ulrich;Simon, Martha N.;Steven, Alasdair C.;Engel, Andreas;Wall, Joseph S.;Aebi, Ueli;Mueller, Shirley A.
关键词：
淀粉样原纤维是丝状蛋白质聚集体，与阿尔茨海默病和 II 型糖尿病等几种常见疾病有关。类似的结构也是传染性海绵状脑病的分子原理，例如人类的克雅氏病、绵羊的痒病，以及所谓的酵母朊病毒（酵母和真菌中发现的遗传性非染色体元素）的分子原理。扫描透射电子显微镜（STEM）通常用于描述淀粉样蛋白聚集体的组装机制和结构特性。在这篇综述中，我们特别考虑了 STEM 对淀粉样蛋白组装途径、原纤维多态性和淀粉样蛋白原纤维结构模型研究的贡献和局限性。这种类型的显微镜提供了直接测量单根细丝的单位长度质量 (MPL) 的唯一方法。对体外组装和离体样品进行的 STEM 质量测量阐明了淀粉样原纤维之间的层次关系，并揭示了多态原纤维和各种球状寡聚体可以从单个多肽同时组装。 MPL 还对可能的堆积方案施加了严格的限制，与固态核磁共振 (NMR) 和电子顺磁共振 (EPR) 等高分辨率方法相结合，有助于分子模型的构建。
Amyloid fibrils are filamentous protein aggregates implicated in several common diseases like Alzheimer’s disease and type II diabetes. Similar structures are also the molecular principle of the infectious spongiform encephalopathies like Creutzfeldt-Jakob disease in humans, scrapie in sheep, and of the so-called yeast prions, inherited non-chromosomal elements found in yeast and fungi. Scanning transmission electron microscopy (STEM) is often used to delineate the assembly mechanism and structural properties of amyloid aggregates. In this review we consider specifically contributions and limitations of STEM for the investigation of amyloid assembly pathways, fibril polymorphisms and structural models of amyloid fibrils. This type of microscopy provides the only method to directly measure the mass-per-length (MPL) of individual filaments. Made on both in vitro assembled and ex vivo samples, STEM mass measurements have illuminated the hierarchical relationships between amyloid fibrils and revealed that polymorphic fibrils and various globular oligomers can assemble simultaneously from a single polypeptide. The MPLs also impose strong constraints on possible packing schemes, assisting in molecular model building when combined with high-resolution methods like solid-state nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR).
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DOI：
10.1038/nsb786
发表时间：
2002-05-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
DePace, AH;Weissman, JS
通讯作者：
Weissman, JS
影响因子：
2.9
作者：
Baxa, Ulrich;Wickner, Reed B.;Tycko, Robert
通讯作者：
Tycko, Robert
DOI：
10.1073/pnas.0706522104
发表时间：
2007-11-27
影响因子：
11.1
作者：
Cobb, Nathan J.;Soennichsen, Frank D.;Surewicz, Witold K.
通讯作者：
Surewicz, Witold K.
影响因子：
0.8
作者：
FRASER, H;DICKINSON, AG
通讯作者：
DICKINSON, AG
影响因子：
3
作者：
Feja, B;Durrenberger, M;Aebi, U
通讯作者：
Aebi, U