Cinnamoyloxy-mammeisin Isolated from Geopropolis Attenuates Inflammatory Process by Inhibiting Cytokine Production: Involvement of MAPK, AP-1, and NF-κB.
Cinnamoyloxy-mammeisin Isolated from Geopropolis Attenuates Inflammatory Process by Inhibiting Cytokine Production: Involvement of MAPK, AP-1, and NF-κB.
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DOI：
10.1021/acs.jnatprod.6b00263
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发表时间：
2016-07-22
影响因子：
5.1
通讯作者：
Cunha TM
中科院分区：
文献类型：
--
作者：
Franchin M;Rosalen PL;da Cunha MG;Silva RL;Colón DF;Bassi GS;de Alencar SM;Ikegaki M;Alves-Filho JC;Cunha FQ;Beutler JA;Cunha TM
Chemical compounds belonging to the class of coumarins have promising anti-inflammatory potential. Cinnamoyloxy-mammeisin (CNM) is a 4-phenylcoumarin that can be isolated from Brazilian geopropolis. To our knowledge, its anti-inflammatory activity has never been studied. Therefore, the present study investigated the anti-inflammatory activity of CNM and elucidated its mechanism of action on isolated macrophages. Pretreatment with CNM reduced neutrophil migration into the peritoneal and joint cavity of mice. Likewise, CNM reduced the in vitro and in vivo release of TNF-α and CXCL2/MIP-2. Regarding the possible molecular mechanism of action, CNM reduced the phosphorylation of proteins ERK 1/2, JNK, p38 MAPK, and AP-1 (subunit c-jun) in PG-stimulated macrophages. Pretreatment with CNM also reduced NF-κB activation in RAW 264.7 macrophages stably expressing the NF-κB-luciferase reporter gene. On the other hand, it did not alter IκBα degradation or nuclear translocation of p65. Thus, the results of this study demonstrate promising anti-inflammatory activity of CNM and provide an explanation of its mechanism of action in macrophages via inhibition of MAPK signaling, AP-1, and NF-κB.
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DOI：
10.1155/2013/907041
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Franchin M;da Cunha MG;Denny C;Napimoga MH;Cunha TM;Bueno-Silva B;Matias de Alencar S;Ikegaki M;Luiz Rosalen P
通讯作者：
Luiz Rosalen P
影响因子：
2.7
作者：
da Cunha, Marcos Guilherme;Rosalen, Pedro Luiz;Beutler, John Albert
通讯作者：
Beutler, John Albert
影响因子：
5.1
作者：
Guo, Weixiao;Sun, Jingjing;Feng, Haihua
通讯作者：
Feng, Haihua
影响因子：
56.9
作者：
AUPHAN, N;DIDONATO, JA;KARIN, M
通讯作者：
KARIN, M
影响因子：
20.1
作者：
BAEZ, S
通讯作者：
BAEZ, S