Vitamin D receptor deficiency and low vitamin D diet stimulate aortic calcification and osteogenic key factor expression in mice.
Vitamin D receptor deficiency and low vitamin D diet stimulate aortic calcification and osteogenic key factor expression in mice.
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DOI：
10.1371/journal.pone.0035316
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Stangl GI
中科院分区：
文献类型：
--
作者：
Schmidt N;Brandsch C;Kühne H;Thiele A;Hirche F;Stangl GI
25-羟基维生素D （25(OH)D）水平低与心血管疾病有关。在此，我们验证了维生素D缺乏可能是动脉粥样硬化性血管改变和血管钙化的一个因果因素的假设。血管钙化染色和成骨分化因子免疫染色的维生素D受体敲除（VDR - / -）小鼠主动脉根切片显示，与野生型小鼠相比，钙化区域更多，成骨关键因子Msx2、Bmp2和Runx2表达量更高（P<0.01）。低密度脂蛋白受体敲除（LDLR - / -）小鼠在16周内被喂食低（50 IU/kg）、推荐（1,000 IU/kg）或高（10,000 IU/kg）维生素D3的西方饮食，数据显示，随着维生素D摄入量的增加，血浆25(OH)D浓度增加（P<0.001），而血浆和股骨中的钙和磷水平不受饮食处理的影响。低维生素D饮食处理的小鼠比喂食推荐量或高维生素D的小鼠主动脉根部有更多的钙化病变和更高的Msx2， Bmp2和Runx2表达（P<0.001）。综上所述，这些发现表明维生素D缺乏是主动脉瓣和主动脉血管钙化的危险因素，也是这些血管区域成骨关键因子表达的刺激因素。
Low levels of 25-hydroxy vitamin D (25(OH)D) are associated with cardiovascular diseases. Herein, we tested the hypothesis that vitamin D deficiency could be a causal factor in atherosclerotic vascular changes and vascular calcification. Aortic root sections of vitamin D receptor knockout (VDR−/−) mice that were stained for vascular calcification and immunostained for osteoblastic differentiation factors showed more calcified areas and a higher expression of the osteogenic key factors Msx2, Bmp2, and Runx2 than the wild-type mice (P<0.01). Data from LDL receptor knockout (LDLR−/−) mice that were fed western diet with either low (50 IU/kg), recommended (1,000 IU/kg), or high (10,000 IU/kg) amounts of vitamin D3 over 16 weeks revealed increasing plasma concentrations of 25(OH)D (P<0.001) with increasing intake of vitamin D, whereas levels of calcium and phosphorus in plasma and femur were not influenced by the dietary treatment. Mice treated with the low vitamin D diet had more calcified lesions and a higher expression of Msx2, Bmp2, and Runx2 in aortic roots than mice fed recommended or high amounts of vitamin D (P<0.001). Taken together, these findings indicate vitamin D deficiency as a risk factor for aortic valve and aortic vessel calcification and a stimulator of osteogenic key factor expression in these vascular areas.
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影响因子：
4.3
作者：
Higashi, Tatsuya;Shibayama, Yujin;Shimada, Kazutake
通讯作者：
Shimada, Kazutake
影响因子：
4
作者：
Speer, Mei Y.;Li, Xianwu;Hiremath, Pranoti G.;Giachelli, Cecilia M.
通讯作者：
Giachelli, Cecilia M.
DOI：
10.1016/0735-1097(90)90282-t
发表时间：
1990-03-15
影响因子：
24
作者：
AGATSTON, AS;JANOWITZ, WR;DETRANO, R
通讯作者：
DETRANO, R
影响因子：
11.1
作者：
Lips, P.;Hosking, D.;Chandler, J.
通讯作者：
Chandler, J.
影响因子：
8.7
作者：
Mori, K;Shioi, A;Morii, H
通讯作者：
Morii, H