Effects of high-pressure treatment on the structure and function of myofibrils.
Effects of high-pressure treatment on the structure and function of myofibrils.
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DOI：
10.2142/biophysico.bppb-v18.010
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发表时间：
2021
影响因子：
1.5
通讯作者：
Shintani SA
中科院分区：
文献类型：
--
作者：
Shintani SA
用高压显微镜观察了高压（40-70 MPa）对肌原纤维结构和功能的影响。当这种压力施加到浸在松弛溶液中的肌原纤维时，肌节长度几乎保持不变，A带变得更短和更宽。施加的压力越高，变化越快。然而，缩短和加宽的A带没有观察到当压力施加到肌原纤维浸渍在通过省略ATP从松弛溶液中获得的溶液。然而，即使在这些条件下，也会发生结构损失，例如Z线结构的损失。为了评估这种压力处理的肌原纤维的结果，诱发并观察肌节的振荡运动（肌节振荡）。即使在结构被破坏的压力处理的肌原纤维中，也有可能诱导肌节振荡。加压降低了肌节振荡的总功率，但没有改变平均频率。在肌节振荡期间，即使施加约40 MPa的压力，平均频率也没有改变。在施加更强的压力以防止观察到肌节振荡之后，当压力恢复到原始值时，平均频率恢复到原始值。这一结果表明，形成横桥的肌球蛋白分子数量的减少并不影响肌节振荡的平均频率。这一事实将有助于建立肌节振荡的力学假说。加压处理是如上所述控制肌原纤维结构的独特方法。
The effects of high pressure (40–70 MPa) on the structure and function of myofibrils were investigated by high pressure microscopy. When this pressure was applied to myofibrils immersed in relaxing solution, the sarcomere length remained almost unchanged, and the A band became shorter and wider. The higher the applied pressure, the faster the change. However, shortening and widening of the A band were not observed when pressure was applied to myofibrils immersed in a solution obtained by omitting ATP from the relaxing solution. However, even under these conditions, structural loss, such as loss of the Z-line structure, occurred. In order to evaluate the consequences of this pressure-treated myofibril, the oscillatory movement of sarcomere (sarcomeric oscillation) was evoked and observed. It was possible to induce sarcomeric oscillation even in pressure-treated myofibrils whose structure was destroyed. The pressurization reduced the total power of the sarcomeric oscillation, but did not change the average frequency. The average frequency did not change even when a pressure of about 40 MPa was applied during sarcomeric oscillation. The average frequency returned to the original when the pressure was returned to the original value after applying stronger pressure to prevent the sarcomere oscillation from being observed. This result suggests that the decrease in the number of myosin molecules forming the crossbridge does not affect the average frequency of sarcomeric oscillation. This fact will help build a mechanical hypothesis for sarcomeric oscillation. The pressurization treatment is a unique method for controlling the structure of myofibrils as described above.
影响因子：
1.5
作者：
Nakagome K;Sato K;Shintani SA;Ishiwata S
通讯作者：
Ishiwata S