Dose-dependent effects of prenatal ethanol exposure on synaptic plasticity and learning in mature offspring.
Dose-dependent effects of prenatal ethanol exposure on synaptic plasticity and learning in mature offspring.
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产前乙醇暴露对成熟后代突触可塑性和学习的剂量依赖性影响。
DOI：
10.1097/01.alc.0000038265.52107.20
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Sutherland,RobertJ
中科院分区：
文献类型：
--
作者：
Savage,DanielD;Becher,Matthew;delaTorre,AlejandroJ;Sutherland,RobertJ
我们已经观察到，母亲在怀孕期间饮用适量乙醇的后代，其海马突触可塑性和一次试验学习能力存在严重缺陷。在本研究中，我们研究了母体血液中较低的乙醇浓度（BECs）是否会导致后代的功能缺陷。方法大鼠在妊娠期间分别饲喂2%、3%或5%乙醇液体饲料。另外三组饲喂0%乙醇的液体饲料，第七组随意饲喂实验室饲料。每个饮食组的成年后代被分配到海马片诱发的[3H]‐D‐天冬氨酸（D‐ASP）释放研究或使用莫里斯水任务进行空间学习研究。结果食用2%、3%和5%乙醇的液体日粮，母体BECs的平均峰值分别为7、30和83 mg/dL。食用这些乙醇饲料对后代的出生体重、产仔数或新生儿死亡率没有影响。同样，从海马切片中诱发的D - ASP释放和莫里斯水任务的标准版本的表现不受产前乙醇暴露的影响。相比之下，在母亲食用5%乙醇液体饮食的后代中，从薄片中诱发的D - ASP释放的活性依赖性增强和莫里斯水任务“移动平台”版本的一次试验学习明显减少。3%乙醇饲粮组的后代在这两个参数上存在中间缺陷，而2%乙醇饲粮组的后代与对照组没有统计学差异。我们得出的结论是，在胎儿期暴露于乙醇环境中，引起细微但显著的学习缺陷的阈值小于30 mg/dL。这个BEC大约相当于每天喝1到1.5盎司的乙醇。
BackgroundWe have observed profound deficits in hippocampal synaptic plasticity and one‐trial learning in offspring whose mothers drank moderate quantities of ethanol during pregnancy. In the present study, we examined the question of whether lower maternal blood ethanol concentrations (BECs) could produce functional deficits in offspring.MethodsRat dams consumed either a 2%, 3%, or 5% ethanol liquid diet throughout gestation. Three other groups of dams were pair‐fed a 0% ethanol liquid diet, and a seventh group consumed lab chow ad libitum. Adult offspring from each diet group were assigned either to studies of evoked [3H]‐D‐aspartate (D‐ASP) release from hippocampal slices or spatial learning studies using the Morris Water Task.ResultsConsumption of the 2%, 3%, and 5% ethanol liquid diets produced mean peak maternal BECs of 7, 30 and 83 mg/dL, respectively. Consumption of these ethanol diets had no effect on offspring birthweight, litter size or neonatal mortality. Likewise, evoked D‐ASP release from hippocampal slices and performance on a standard version of the Morris Water Task were not affected by prenatal ethanol exposure. By contrast, activity‐dependent potentiation of evoked D‐ASP release from slices and one‐trial learning on a “moving platform” version of the Morris Water Task were markedly reduced in offspring whose mothers consumed the 5% ethanol liquid diet. Intermediate deficits in these two parameters were observed in offspring from the 3% ethanol diet group, whereas offspring from the 2% ethanol diet group were not statistically different than controls.ConclusionsWe conclude that the threshold for eliciting subtle, yet significant learning deficits in offspring prenatally exposed to ethanol is less than 30 mg/dL. This BEC is roughly equivalent to drinking 1 to 1.5 ounces of ethanol per day.
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DOI：
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发表时间：
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